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AP Biology Free Response Questions

The best way to get better at FRQs is practice. Browse through dozens of practice AP Biology FRQs to get ready for the big day.

  • View all (250)
  • Unit 1: Chemistry of Life (22)
  • Unit 2: Cell Structure and Function (25)
  • Unit 3: Cellular Energetics (24)
  • Unit 4: Cell Communication and Cell Cycle (29)
  • Unit 5: Heredity (39)
  • Unit 6: Gene Expression and Regulation (22)
  • Unit 7: Natural Selection (62)
  • Unit 8: Ecology (27)
Unit 1: Chemistry of Life

Enzymatic Activity and Protein Structure

Enzymes are proteins whose structures are critical to their catalytic function. Examine how environm

Medium

Enzyme Activity and Environmental pH

Analyze how changes in pH affect enzyme activity and structure.

Medium

Evaluating the Relationship Between Molecular Structure and Biological Function

This FRQ challenges you to examine how the structure of various organic molecules determines their b

Extreme

FRQ 2: Subatomic Particles and Isotopes

Explain the structure of an atom by discussing the roles of protons, neutrons, and electrons, and il

Medium

FRQ 18: Dehydration Synthesis in Proteins versus Carbohydrates

Compare dehydration synthesis reactions in the formation of proteins and carbohydrates, highlighting

Extreme

Hydrogen Bonding in Water: A Molecular Perspective

A chemist is investigating the unique properties of water that arise from hydrogen bonding. Answer t

Hard

Lipids and Membrane Structure

Discuss the roles of phospholipids and cholesterol in maintaining membrane fluidity and integrity, a

Medium

Lipids: Structure and Function in Cell Membranes

Discuss the various types of lipids found in biological membranes and their significance in cellular

Easy

Macromolecule Synthesis and Breakdown

In a biology laboratory, scientists are exploring how large biomolecules are built and degraded. Ans

Medium

Nucleic Acid Stability Under Temperature Variation

A geneticist investigates the thermal stability of DNA by monitoring its denaturation as the tempera

Medium

Nucleic Acids and Genetic Information

Evaluate the structures of nucleic acids and discuss how these structures enable them to perform the

Easy

Organic Molecules and Functional Groups

Examine how functional groups in organic molecules influence their chemical reactivity and biologica

Easy

Organic Molecules: Dehydration Synthesis and Hydrolysis

Examine the processes of dehydration synthesis and hydrolysis in the formation and breakdown of poly

Medium

Protein Structure Analysis

A research lab has isolated a novel protein and characterized its structures using biochemical metho

Hard

Protein Structure and Function

Examine the four levels of protein structure and explain how the primary sequence of amino acids inf

Medium

Protein Structure and Mutation Analysis

A molecular biologist is investigating the structure of proteins and the effects of mutations.

Medium

Subatomic Particles and Elemental Diversity

Discuss the fundamental components of atoms and explain the significance of isotopic variation in bi

Easy

Subatomic Structure and Isotope Variation in Atoms

This FRQ focuses on atomic structure and isotopic differences. You will describe the arrangement of

Medium

Trace Elements and Enzyme Activity

A biochemist hypothesizes that trace elements, such as copper (Cu), affect the activity of a specifi

Medium

Trace Elements in Ecosystem Function

An ecologist is researching the role of trace elements (e.g., iron, iodine, copper) in the health of

Medium

Water in Polymer Hydrolysis Reactions

Analyze the role of water in the hydrolysis of polymers.

Medium

Water: Unique Properties and Capillary Action

Water exhibits several unique physical properties that are crucial for life. Analyze how these prope

Medium
Unit 2: Cell Structure and Function

Cell Diffusion Efficiency Based on Radius

A researcher measured the diffusion rates of a solute in cells of varying radii. The theoretical rel

Medium

Comparative Analysis of Plant and Animal Cells

Plant and animal cells exhibit distinct structural features that reflect their functions. Answer the

Easy

Comparative Analysis of Prokaryotic and Eukaryotic Cells

The following table lists key features of prokaryotic and eukaryotic cells. Analyze the differences

Medium

Comparing Passive and Active Transport under Variable Conditions

A study compares the efficiency of passive (diffusion) and active transport mechanisms under varying

Hard

Cytoskeleton Function and Cellular Dynamics

The cytoskeleton is essential for maintaining cell shape, enabling movement, and facilitating cell d

Hard

Cytoskeleton Structural Stability Experiment

Researchers conducted an experiment to determine the role of the cytoskeleton in maintaining cell sh

Medium

Dialysis and Solute Diffusion Analysis

This FRQ explores how the principles of diffusion are utilized in dialysis to remove solutes from th

Easy

Differences Between Plant and Animal Cells

Plant and animal cells share many features but also display distinct differences. Analyze these diff

Easy

Energy Conversion in Mitochondria vs. Peroxisomes

This question contrasts energy conversion efficiencies between mitochondria and peroxisomes and disc

Extreme

Golgi Apparatus Function Analysis

An experiment was designed to study the role of the Golgi apparatus in processing and packaging prot

Medium

Kinetics of Receptor-Mediated Endocytosis

This question integrates data analysis with kinetic concepts to understand receptor-mediated endocyt

Extreme

Membrane Transport: Diffusion and Osmosis

This question examines membrane transport through calculations using the water potential equation an

Medium

Microscopy and its Applications in Cell Biology

Microscopy is a fundamental tool for exploring cell structure. Compare and contrast light microscope

Medium

Microscopy in Cellular Biology

This question explores the application of different microscopy techniques in cellular biology.

Easy

Microscopy: Light versus Electron

This question investigates the advantages and limitations of different microscopic techniques in stu

Medium

Mitochondrial Function and Energy Production

This question focuses on the role of mitochondria in energy production and the relationship between

Easy

Mitochondrial Structure and ATP Production Efficiency

An experimental physiologist is studying mitochondria to understand how their structure contributes

Hard

Nuclear Organization and Function

The nucleus is central to eukaryotic cell function. (a) Explain the role of the nucleus in gene reg

Medium

Osmosis and Tonicity in Plant Cells

A graph of experimental data shows the relationship between external solute concentration and turgor

Hard

Osmosis and Water Potential in Cells

This question assesses understanding of osmosis, water potential, and the consequences of solute con

Hard

Plasma Membrane Structure and Function

A cell biologist investigates how the composition of the plasma membrane affects its transport funct

Medium

Prokaryotic vs Eukaryotic Cellular Organization

Answer the following questions comparing prokaryotic and eukaryotic cells. (a) List and explain thr

Medium

Role of Plasma Membrane in Selective Permeability

A series of experiments using artificial membranes have aimed to test the claim that the plasma memb

Medium

Sodium-Potassium Pump: Active Transport and Energy Consumption

A biochemist is investigating the sodium-potassium pump in animal cells. The pump moves 3 Na+ ions o

Medium

Transport Proteins Functionality: Channels vs Pumps

This FRQ examines the differences between passive transport via channel proteins and active transpor

Medium
Unit 3: Cellular Energetics

ATP Yield in Cellular Respiration

A student analyzes the stages of cellular respiration to determine the ATP yield from glycolysis, py

Medium

Cellular Respiration Stages and Energy Production

Describe the four stages of cellular respiration, detailing the key reactions and net production of

Hard

Chemiosmosis and Proton Gradient Analysis

Explain the process of chemiosmosis and analyze how the proton gradient is generated and utilized in

Medium

Coenzymes and their Roles in Enzymatic Reactions

A researcher is investigating the impact of supplementing a coenzyme on the activity of an enzyme in

Medium

Cofactors and Coenzymes in Enzyme Function

Discuss the roles of cofactors and coenzymes in enzyme-catalyzed reactions and provide an example hi

Easy

Comparative ATP Production in Cellular Respiration vs Photosynthesis

A researcher compares energy production in heterotrophic cells via cellular respiration and in autot

Extreme

Designing an Experiment on Allosteric Regulation

Design a controlled experiment to test the effect of an allosteric inhibitor on enzyme activity.

Hard

Electron Transport and ATP Production Correlation

A graph is provided showing the relationship between the proton gradient and ATP production. Analyze

Medium

Electron Transport Chain (ETC) and Oxidative Phosphorylation

This question explores the mechanism of the electron transport chain and oxidative phosphorylation i

Hard

Energy Conversion Comparisons: Photosynthesis vs. Cellular Respiration

Design an experiment to compare the energy conversion efficiency of photosynthesis and cellular resp

Hard

Energy Diagrams Analysis

Analyze the energy diagram provided to determine the type of reaction depicted. Use the graph to ext

Medium

Enzyme Kinetics Analysis

A researcher conducts an experiment where the rate of an enzyme reaction is measured at varying subs

Easy

Evaluation of Light Wavelength Effects on Photosynthetic Pigment Absorption

A group of researchers aimed to determine the absorption spectra of various photosynthetic pigments

Hard

Impact of Allosteric Inhibitors on Enzyme Regulation

A table below shows enzyme reaction rates in the presence of various concentrations of an inhibitor.

Hard

Impact of pH on ATP Hydrolysis Rate

Review the table below showing rates of ATP hydrolysis at various pH levels. Analyze the data to det

Easy

Impact of pH on Photosynthetic Efficiency

Design an experiment to investigate the effect of pH on the rate of photosynthesis in aquatic plants

Medium

pH Effects on Enzyme Function

This question investigates how pH influences enzyme structure and function.

Easy

Reaction Coupling and ATP Hydrolysis

Cells drive endergonic reactions by coupling them with exergonic ones such as the hydrolysis of ATP.

Hard

Reaction Coupling in Metabolic Pathways

Define reaction coupling in cellular metabolism and design a hypothetical pathway where it is essent

Extreme

Regulation of Metabolic Pathways in Cellular Respiration

This question explores the regulatory mechanisms controlling cellular respiration, including feedbac

Hard

Temperature and pH Effects on Enzyme Activity

The activity of enzymes is highly dependent on environmental conditions. Using the provided data tab

Easy

Temperature Dependence of Enzyme Activity

A researcher measured an enzyme's activity at various temperatures, and the results are shown in the

Medium

The Calvin Cycle and Carbon Fixation

This question examines the biochemical steps of the Calvin cycle and its regulation in different pla

Hard

Thermodynamics in Coupled Metabolic Reactions

This question challenges you to integrate thermodynamic principles with the concept of reaction coup

Extreme
Unit 4: Cell Communication and Cell Cycle

Analyzing Signal Transduction Pathways: Crosstalk and Amplification

An experiment investigates the dynamics of a signal transduction cascade and suggests there may be c

Hard

Apoptosis Trigger Mechanisms in Damaged Cells

Cells exposed to high doses of radiation show evidence of programmed cell death (apoptosis). An inve

Hard

Bacterial Chemotaxis Investigation

A scientist is investigating the chemotactic behavior of a unicellular bacterium in response to vary

Easy

Cell Cycle Disruption and Cancer Development

Mutations affecting cell cycle regulators can lead to cancer. Answer the following questions regardi

Hard

Cell Cycle Dysregulation and Apoptosis

Failure of cell cycle checkpoints can lead to uncontrolled cell division and trigger programmed cell

Hard

Cell Division Regulation: Unicellular vs. Multicellular Organisms

Cell division is regulated differently in unicellular and multicellular organisms. Part a: Compare

Medium

Chemotaxis and Signal Transduction in Unicellular Organisms

This question examines how unicellular organisms use chemotaxis to detect and respond to environment

Medium

Comparative Analysis of Membrane Receptor Classes

Cells utilize various classes of membrane receptors to perceive and respond to external signals. Con

Hard

Comparative Analysis of Membrane Receptors

Various classes of membrane receptors differ in structure and function. A researcher is comparing li

Medium

Comparative Analysis of Mitosis and Cytokinesis

A study compares the process of mitosis in two different cell types—one with rapid division and anot

Hard

Comparative Signal Transduction in Cancerous vs. Normal Cells

Construct an experimental plan to compare the activation of signal transduction pathways in cancerou

Extreme

Cyclin-Dependent Kinases and Cell Cycle Progression

An experiment measured cyclin concentrations during different phases of the cell cycle. The data bel

Hard

Effects of Toxins on Receptor Function

Certain toxins act as competitive inhibitors by binding to the ligand-binding sites of membrane rece

Medium

G-Protein Coupled Receptor Dynamics

Researchers are investigating the impact of a single amino acid mutation in a G-protein coupled rece

Hard

Impact of Environmental Stimuli on Unicellular Organisms

Unicellular organisms rely on taxis to navigate their environments. Answer the following questions r

Easy

Integration of Cell Communication and Cell Cycle Control in Tissue Repair

A researcher is studying wound healing in epithelial tissue and observes that cell communication thr

Hard

Mathematical Modeling of Diffusion in Signal Transduction

This question explores the diffusion of signaling molecules using Fick's First Law and requires a mu

Hard

Mitosis and Chromosome Segregation

This question focuses on the stages of mitosis and the mechanisms that ensure accurate chromosome se

Easy

Negative Feedback in Homeostasis: Regulation of Blood Glucose

This question explores homeostatic regulation through negative feedback mechanisms, focusing on bloo

Easy

Receptor Binding and Signal Specificity

A research group examines signal transduction through enzyme-linked receptors in cultured fibroblast

Hard

Regulation of the Cell Cycle: Cyclins and CDKs

This question focuses on cyclins and cyclin-dependent kinases (CDKs) and their roles in regulating t

Medium

Role of G-protein Coupled Receptors in Cell Signaling

G-protein Coupled Receptors (GPCRs) play a crucial role in transmitting external signals into a cell

Medium

Signal Amplification in Cellular Communication

A cell receives a low concentration of a signaling molecule, yet the resulting intracellular respons

Medium

Signal Transduction and Metabolic Enzyme Regulation

This question explores the interplay between signal transduction pathways and metabolic regulation,

Medium

Signal Transduction Cascade Analysis

A researcher is analyzing the signal transduction cascade initiated by ligand binding to a plasma me

Medium

Signal Transduction in Immune Response Activation

A study examines the activation levels of immune cells following exposure to a pathogen-associated m

Medium

Signal Transduction Pathway Analysis in Eukaryotic Cells

A research lab studies a hormone signaling pathway in eukaryotic cells. In this pathway, the hormone

Medium

Temporal Coordination of DNA Replication and the S Phase

Accurate DNA replication during the S phase is essential for genomic stability. Evaluate the timing

Hard

Visual Analysis of a Cell Cycle Schematic

A schematic diagram of the cell cycle has been provided. Analyze this diagram in detail.

Hard
Unit 5: Heredity

Application of Probability Rules in Genetics

A plant with genotype RrYy produces gametes following independent assortment. If the probability of

Hard

Assessing Genetic Variation through Test Cross Design

A researcher conducted a test cross by mating a dominant phenotype plant with a recessive phenotype

Easy

Assessing Recombination Frequency in Linked Genes

A geneticist designed an experiment to determine the recombination frequency among three linked gene

Hard

Barr Bodies and X-Inactivation

X-inactivation is a key mechanism to ensure dosage compensation in females. Describe the process of

Medium

Chromosomal Nondisjunction and Developmental Defects

Errors during meiosis can lead to nondisjunction, resulting in abnormal chromosome numbers (aneuploi

Hard

Comparative Analysis of Inheritance Patterns

Modern genetics recognizes both Mendelian and non‐Mendelian patterns of inheritance. Compare and con

Extreme

Comparative Analysis of Spermatogenesis and Oogenesis

A reproductive biologist is comparing the processes of spermatogenesis and oogenesis to understand t

Easy

Comparative Analysis: Spermatogenesis versus Oogenesis

Spermatogenesis and oogenesis are two distinct processes of gamete formation in animals. Each proces

Easy

Comparing Mitosis and Meiosis

Cell division occurs via mitosis and meiosis, each serving distinct purposes. Answer the following:

Medium

Contrasting Inheritance Patterns: Polygenic, Codominance, and Incomplete Dominance

Discuss how polygenic inheritance, codominance, and incomplete dominance differ in their effects on

Extreme

Critiquing an Experimental Protocol on Linked Genes Mapping

A genetics laboratory designed an experiment to map linked genes on a chromosome by calculating reco

Hard

Determining Genotypes through Test Crosses

A test cross was performed by crossing a tall plant of unknown genotype with a short, homozygous rec

Easy

Differences Between Meiosis I and II

Meiosis is divided into two successive divisions, each with unique characteristics. Compare and cont

Hard

Environmental Influence on Phenotypic Plasticity

Environmental factors can influence the phenotype even when the genotype remains constant. This phen

Medium

Epigenetics and the Influence of the Environment

Environmental factors can alter gene expression through epigenetic modifications. Answer the followi

Medium

Evaluating Environmental Effects on Phenotypic Plasticity in Plants

Design an experiment to assess the impact of environmental conditions on the phenotype of geneticall

Hard

Examining Dihybrid Cross and Independent Assortment in Pea Plants

Design an experiment using a dihybrid cross in pea plants to test Mendel’s Law of Independent Assort

Medium

Examining Meiotic Nondisjunction and Its Consequences in Model Organisms

Design an experiment using a model organism (e.g., fruit flies or yeast) to study the effects of non

Extreme

Experimental Design in Genetics: Detecting Linked Genes

Designing experiments to determine whether two genes are linked requires careful planning and analys

Extreme

Exploring X-Chromosome Inactivation and Barr Bodies

Researchers study female mammals to understand X-chromosome inactivation. They observe the presence

Easy

Gamete Production and Fertilization

Describe the process of gametogenesis in males and females and analyze how these processes contribut

Medium

Gene Mapping and Crossing-Over Analysis

In a genetic mapping experiment, a researcher obtained the following recombination frequencies: | G

Extreme

Haploid and Diploid Cells

Many organisms have both haploid and diploid cells. In your answer, describe the differences between

Easy

Haploid vs Diploid Analysis and Gamete Formation

A researcher is investigating the fundamentals of cell division and gametogenesis. The study compare

Easy

Incomplete Dominance in Snapdragon Flowers

When a red-flowered snapdragon (genotype RR) is crossed with a white-flowered snapdragon (genotype W

Easy

Interplay Between Mendelian and Non-Mendelian Inheritance

A series of crosses were performed demonstrating both Mendelian traits and non-Mendelian inheritance

Medium

Interpreting Pedigree Data

Given the following simplified pedigree data for a trait that appears to be recessive: | Generation

Medium

Investigating Gene Expression and Pedigree Data for Sex-linked Conditions

Analyze the provided pedigree data for a sex-linked condition (e.g., hemophilia) and predict inherit

Hard

Maternal Inheritance of Mitochondrial Traits in Plants

Design an experiment to investigate the maternal inheritance of mitochondrial traits in a plant spec

Medium

Meiosis: Stages and Their Significance

Meiosis is critical for gamete formation and genetic diversity. Describe the key differences between

Medium

Mendelian Genetics and the Law of Dominance

Gregor Mendel’s experiments with pea plants provided the foundation for classical genetics. Consider

Medium

Non-Mendelian Inheritance: Codominance in Flower Color

A researcher is investigating flower color in a plant species known to have codominant alleles for r

Easy

Pedigree Analysis and Carrier Probability

In a pedigree study of an autosomal recessive trait, individual III-2 has an affected sibling while

Hard

Punnett Square Analysis of Sex-Linked Crosses

Consider a cross between a carrier female (X^N X^n) and a normal male (X^N Y) for an X-linked recess

Medium

Recombinant Frequencies and Genetic Map Construction

Using recombination frequencies is a standard method for constructing genetic maps. In this scenario

Extreme

Revisiting Mendel's Laws

Review Mendel's three principles of inheritance—the Law of Dominance, the Law of Segregation, and th

Easy

Sex-Linked Inheritance in Pedigree Data

A geneticist analyzed a set of pedigree data for a trait suspected to be X-linked. The table below s

Medium

Sex-linked Trait Analysis using a Pedigree

A genetic counselor analyzed a pedigree chart to study the inheritance of a sex-linked trait within

Medium

Test Cross Analysis for Genotype Determination

A test cross is used to determine the genotype of an individual with a dominant phenotype. In this s

Medium
Unit 6: Gene Expression and Regulation

Advances in DNA Sequencing Techniques

Examine Sanger sequencing and next-generation sequencing technologies, and compare their respective

Medium

Chromatin Remodeling and Epigenetic Regulation Experiment

A researcher treats cultured cells with a histone deacetylase (HDAC) inhibitor to study its effects

Hard

Comparative Structures of DNA and RNA

DNA and RNA have distinct structural differences that underlie their different biological functions.

Easy

DNA Double Helix Structure Interpretation

Examine the attached diagram of a DNA double helix and answer the following parts regarding its stru

Easy

DNA Packaging and Histone Modifications

Chromatin structure, including histone modifications, plays a critical role in regulating gene expre

Medium

DNA Polymerase Proofreading and Replication Fidelity

An in vitro experiment compares DNA replication by polymerases with and without proofreading activit

Easy

DNA Replication Dynamics and Enzyme Function

A research laboratory is investigating the roles of various enzymes in DNA replication. Using the pr

Hard

DNA Replication Efficiency Experiment

A researcher is investigating the effect of DNA polymerase concentration on the rate of DNA replicat

Medium

DNA Replication Kinetics

This question explores the kinetics of DNA replication. Imagine a cell where each replication fork s

Medium

Effects of DNA Mutations on Protein Structure

An enzyme activity assay is conducted on proteins translated from genes harboring different point mu

Medium

Effects of Inducers on the lac Operon Expression

Design a controlled experiment to assess the impact of varying lactose concentrations on the express

Medium

Effects of Mutations on Protein Function

Analyze different types of point mutations and discuss their potential impacts on protein synthesis.

Medium

Energy Consumption in DNA Replication by Helicase

Helicase unwinds the DNA double helix during replication. Suppose unwinding 1 kilobase (kb) of DNA r

Hard

Exploring the Role of Telomerase in Telomere Length Maintenance

Design an experiment to investigate the effects of telomerase activity on telomere length in culture

Hard

FRQ 5: Translation and Protein Synthesis

An mRNA transcript carrying a mutation in its start codon is introduced into an in vitro translation

Medium

Gel Electrophoresis and RFLP Analysis

Answer the following questions related to gel electrophoresis and restriction fragment length polymo

Medium

Hox Genes and Embryonic Development

Analyze the role of Hox genes in embryonic development and the impact of their mutation on organisma

Hard

Impacts of Genetic Mutations on Protein Synthesis

Considering different types of genetic mutations, answer the following questions regarding their eff

Medium

Operon Function and Regulation

Using the provided operon diagram, answer the following questions concerning the regulation of gene

Medium

Regulation of Gene Expression in Prokaryotes and Eukaryotes

Compare the mechanisms of gene regulation in prokaryotes and eukaryotes, highlighting both shared pr

Medium

Semiconservative Replication and DNA Parental Strands

Semiconservative replication results in daughter DNA molecules that each contain one parental strand

Medium

Transcription Rate Under Promoter Mutations

A geneticist is investigating the impact of promoter mutations on transcription efficiency. The tabl

Medium
Unit 7: Natural Selection

Abiotic Selective Pressures in an Arid Environment

In an arid region experiencing prolonged drought, a native plant species faces severe water scarcity

Hard

Adaptive Traits and Finch Beak Variation

A population of finches exhibits variation in beak size that correlates with environmental condition

Easy

Artificial Selection and Crop Improvement

Agricultural scientists use artificial selection to improve traits such as yield in wheat crops. Dat

Medium

Artificial Selection Data Analysis

A table provides data on a trait (e.g., beak size in birds) assessed over several generations under

Medium

Artificial vs. Natural Selection

This question compares artificial and natural selection. Design an experiment and analyze the evolut

Medium

Biogeographical Patterns and Evolution

Biogeography provides key evidence for evolution by examining the distribution of species across geo

Medium

Comparative Analysis of Convergent and Divergent Evolution

Define and compare convergent and divergent evolution, and discuss how these processes are driven by

Easy

Comparative Analysis of Morphological Homologies in Vertebrates

Discuss the significance of morphological homologies in understanding evolutionary relationships amo

Easy

Comparative Embryology and Evolutionary Development

Discuss how comparative embryology supports evolutionary theory by examining similarities and differ

Medium

Comparing Evolutionary Theories: Darwin vs. Lamarck

Answer the following questions comparing two evolutionary theories:

Medium

Constructing a Phylogenetic Tree

Explain the process of constructing a phylogenetic tree using molecular data. Answer the following p

Easy

Constructing and Interpreting Phylogenetic Trees

A phylogenetic analysis yields a graphical representation of evolutionary relationships among severa

Extreme

Darwin vs. Lamarck: Mechanisms of Evolution

The theories proposed by Charles Darwin and Jean-Baptiste Lamarck offer different explanations for h

Medium

Darwin's Observations and Natural Selection

A researcher reviews historical data and observations that contributed to Darwin's formulation of na

Medium

Designing an Experiment on Environmental Impact on Evolution

Design a controlled experiment to test how a specific abiotic factor influences the rate of evolutio

Hard

Ecological Interactions Driving Evolution

Population dynamics of predators and prey can exert selective pressures that drive evolutionary chan

Medium

Embryological Evidence for a Common Ancestry

Embryological studies, including the presence of gill slits in vertebrate embryos, have been used as

Hard

Environmental Pressure and Enzyme Activity

A study investigates how enzyme activity in an organism changes with temperature. A graph depicting

Hard

Environmental Pressures and Phenotypic Traits

A study examines the effect of temperature on the beak size of a particular bird species. Use the pr

Medium

Enzyme Activity Variation in Fish Gill Cells under Different pH Conditions

In this experiment, a researcher isolates gill cells from a fish species and measures the activity o

Medium

Evaluating Experimental Methods in Evolution Research

Evaluate a media claim regarding rapid evolution by analyzing experimental methods used in studying

Extreme

Evaluating Inheritance Theories: Lamarck vs Darwin

In a laboratory simulation, two populations of snails were observed over 10 generations. One populat

Hard

Evaluating Mutation Effects on Hardy-Weinberg Equilibrium

This FRQ examines how an increase in mutation rates can disturb Hardy-Weinberg equilibrium in a popu

Medium

Evolution of Enzyme Function in Variable Environments

A study examines the catalytic activity of an enzyme extracted from a plant species and how it chang

Extreme

Evolutionary Fitness and Changing Interactions

Discuss the concept of evolutionary fitness and analyze how changing ecological interactions, such a

Medium

Experimental Design in Artificial Selection: Domestication Traits

This FRQ examines the principles of artificial selection in the context of domestication. Consider h

Easy

Factors Disrupting Hardy-Weinberg Equilibrium

Discuss the factors that can disrupt Hardy-Weinberg equilibrium in a population:

Medium

Fossil Evidence and Phylogenetic Trees

Paleontologists use fossil records, radiometric dating, and cladistics to study evolutionary relatio

Hard

Gene Flow and Its Impact on Population Genetics

Gene flow plays a critical role in shaping genetic diversity within populations. Examine how migrati

Medium

Gene Flow and Local Adaptation

Examine how immigration and emigration (gene flow) affect allele frequencies and influence local ada

Extreme

Hardy-Weinberg Equilibrium and Population Genetics

A biologist studies a large, randomly mating population under conditions presumed to follow Hardy-We

Medium

Integrative Analysis of Evolutionary Mechanisms

Evolution is driven by multiple mechanisms that can interact simultaneously. Answer the following:

Extreme

Interpreting Phylogenetic Trees and Common Ancestry

Analyze a simplified phylogenetic tree to infer evolutionary relationships among vertebrate species

Medium

Lamarck vs Darwin: Mechanisms of Evolution

Evaluate the differences between Lamarck’s theory of acquired traits and Darwin’s theory of natural

Medium

Lamarckian vs Darwinian Evolution

In a laboratory experiment, a population of unicellular organisms is exposed to a novel stressor. Ov

Medium

Mechanisms of Speciation and Reproductive Isolation

Examine the different mechanisms of speciation and the role of reproductive isolation in the evoluti

Medium

Mendelian Inheritance and Hardy-Weinberg Equilibrium Misinterpretation

In this experiment, a researcher studies the genetic structure of a small beetle population by genot

Medium

Miller-Urey Experiment and Organic Compounds

Evaluate the significance of the Miller-Urey experiment in understanding the origin of life on Earth

Medium

Miller-Urey Experiment and the Origin of Organic Molecules

In an experiment simulating early Earth conditions, scientists introduced a mixture of gases, applie

Medium

Molecular Evidence and Evolutionary Relationships

Examine how molecular data, such as DNA or protein sequences, provide evidence for common ancestry a

Medium

Molecular Evidence for Common Ancestry

A research study compares the genetic sequence of a particular gene across several mammalian species

Hard

Morphological Homologies vs. Analogous Structures

Differentiate between morphological homologies and analogous structures and explain their significan

Medium

Mutation, Gene Flow, and Genetic Drift in Evolution

Discuss the roles of mutation, gene flow, and genetic drift as mechanisms that generate genetic vari

Medium

Mutation, Genetic Drift, and Evolutionary Change

Mutations, genetic drift, and gene flow are mechanisms that contribute to genetic variation. Analyze

Hard

Natural Selection Under Changing Environmental Conditions

Analyze how environmental changes impact allele frequencies in a population based on provided experi

Hard

Natural Selection vs Genetic Drift

In a small isolated population, both natural selection and genetic drift can affect allele frequenci

Hard

Peppered Moth Case Study: Data Interpretation

A table presents data on the phenotypic ratios of dark and light peppered moths before and after ind

Medium

Peppered Moths and Directional Selection

Examine how environmental changes influenced natural selection in peppered moths. Answer the followi

Medium

Peppered Moths: An Example of Natural Selection

This question examines the effects of natural selection on phenotype frequencies using the example o

Medium

Population Bottlenecks and Founder Effects

Population bottlenecks and founder effects are important examples of genetic drift. Evaluate how the

Medium

Population Bottlenecks and Genetic Drift

Population bottlenecks can profoundly influence genetic diversity through genetic drift. Consider th

Hard

Reproductive Isolation in Speciation

Discuss the role of reproductive isolation mechanisms in the speciation process.

Medium

Selective Pressures in Plant Populations: Light and Resource Competition

An experiment is conducted on two groups of plants grown under varying light intensities. Researcher

Medium

Sexual Selection and Evolutionary Dynamics

Analyze the role of sexual selection in evolution and discuss the cost-benefit trade-offs associated

Medium

Sexual Selection and Evolutionary Trade-Offs

Examine the role of sexual selection in evolution and its potential trade-offs:

Medium

Sexual Selection and Trait Exaggeration

The table below records data on male bird tail lengths and their corresponding number of mating part

Medium

Sexual Selection and Trait Variation

A study explores the relationship between male tail length and mating success. The graph provided sh

Hard

Simulating Early Earth: The Miller-Urey Experiment

The Miller-Urey experiment attempted to simulate early Earth conditions and resulted in the formatio

Medium

Testing Differential Reproductive Success

This FRQ challenges you to design a rigorous experiment investigating how variation in a specific tr

Extreme

Testing the RNA-World Hypothesis with Laboratory Simulations

This FRQ investigates the RNA-world hypothesis by asking you to design a detailed experimental proto

Extreme

The RNA World Hypothesis

Discuss the RNA world hypothesis and its significance in explaining the origins of life on Earth.

Medium

The Role of Sexual Selection in Trait Evolution

Sexual selection is a powerful evolutionary force often driving dramatic trait exaggeration. Using t

Medium
Unit 8: Ecology

Altruistic Behavior and Group Dynamics

Altruistic behavior, in which an individual benefits another at a cost to itself, poses interesting

Medium

Altruistic Behavior and Group Fitness in Social Insects

In some social insects, altruistic behavior by individuals can increase overall colony survival desp

Extreme

Altruistic Behavior and Kin Selection

A behavioral ecologist investigates altruistic behavior in social mammals with a focus on kin select

Hard

Analyzing Population Growth Using the r Equation

A researcher studying an insect population collected data on births and deaths over one generation a

Easy

Animal Communication: Pheromones and Agonistic Behaviors

This FRQ focuses on the various ways animals communicate, emphasizing chemical signaling and social

Easy

Carrying Capacity and Population Regulation

This final question examines the concept of carrying capacity and how external factors can regulate

Medium

Carrying Capacity and Population Stability

An ecologist monitors a population of rodents in a confined habitat. The data initially show exponen

Medium

Chemical Communication Analysis

A study was conducted to analyze how varying concentrations of pheromones affect the frequency of a

Medium

Climate Change Effects on Population Dynamics and Distribution

Assess the potential impacts of climate change on species' population growth and geographic distribu

Extreme

Differentiating Gravitropism and Thigmotropism in Climbing Plants

Researchers studied climbing plants’ responses to gravitational and touch stimuli, claiming that gra

Hard

Ecological Pyramid and Energy Transfer

Analyze the provided table displaying energy values across different trophic levels in an ecosystem.

Medium

Ecological Pyramids and Energy Transfer Efficiency

In many ecosystems, only about 10% of energy is transferred from one trophic level to the next. Cons

Medium

Ecological Succession in a Post-Volcanic Landscape

Investigate the process of primary ecological succession following a volcanic eruption, focusing on

Medium

Ecosystem Analysis of Keystone Species

An ecosystem study involved the experimental removal of a keystone species. Data on species populati

Extreme

Evaluating Mutualistic Symbiosis

Examine a symbiotic relationship between a fungus and an alga that forms lichen. Data on growth rate

Hard

Exponential Growth in Bacterial Cultures

A bacterial culture starts with 100 cells and doubles every 3 hours. The growth is modeled by the eq

Easy

Habituation Response Analysis

In an experiment designed to study habituation, an animal's response magnitude to a repeated stimulu

Medium

Human Impact: Deforestation and Biodiversity Loss

A conservation biologist is examining the effects of deforestation on species diversity in tropical

Extreme

Instinct, Learning, and Imprinting in Birds

A researcher studies the behavior of ducklings with respect to imprinting. In the absence of their m

Medium

Interactions of Endothermy and Behavior

A researcher examines energy expenditure in birds by comparing endothermic metabolic costs and the i

Hard

Photoperiodism and Flowering in Plants

Photoperiodism is a critical factor influencing the reproductive cycle in many plants. This FRQ expl

Medium

Photoperiodism and Plant Flowering

Plants rely on photoperiodism—the response to the duration of day and night—to time essential proces

Hard

Plant Tropisms: Phototropism and Gravitropism

In an experiment, a researcher exposes seedlings to directional light and altered gravitational cond

Medium

Population Dynamics Calculation

A monitored animal population provided data on births, deaths, and total population size over time.

Medium

Primary Productivity: Gross vs. Net

A researcher measured both the gross productivity and respiration rate of a photosynthetic community

Extreme

Simpson’s Diversity Index Calculation

An environmental scientist collects data on species in a forest plot: Species A - 40 individuals, Sp

Hard

Symbiotic Relationships: Mutualism, Commensalism, and Parasitism

Symbiotic relationships define the interactions between different species living in close associatio

Hard

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Where can I find practice free response questions for the AP Biology exam?
The free response section of each AP exam varies slightly, so you’ll definitely want to practice that before stepping into that exam room. Here are some free places to find practice FRQs :
  • Of course, make sure to run through College Board's past FRQ questions!
  • Once you’re done with those go through all the questions in the AP BiologyFree Response Room. You can answer the question and have it grade you against the rubric so you know exactly where to improve.
  • Reddit it also a great place to find AP free response questions that other students may have access to.
How do I practice for AP AP Biology Exam FRQs?
Once you’re done reviewing your study guides, find and bookmark all the free response questions you can find. The question above has some good places to look! while you’re going through them, simulate exam conditions by setting a timer that matches the time allowed on the actual exam. Time management is going to help you answer the FRQs on the real exam concisely when you’re in that time crunch.
What are some tips for AP Biology free response questions?
Before you start writing out your response, take a few minutes to outline the key points you want to make sure to touch on. This may seem like a waste of time, but it’s very helpful in making sure your response effectively addresses all the parts of the question. Once you do your practice free response questions, compare them to scoring guidelines and sample responses to identify areas for improvement. When you do the free response practice on the AP Biology Free Response Room, there’s an option to let it grade your response against the rubric and tell you exactly what you need to study more.
How do I answer AP Biology free-response questions?
Answering AP Biology free response questions the right way is all about practice! As you go through the AP AP Biology Free Response Room, treat it like a real exam and approach it this way so you stay calm during the actual exam. When you first see the question, take some time to process exactly what it’s asking. Make sure to also read through all the sub-parts in the question and re-read the main prompt, making sure to circle and underline any key information. This will help you allocate your time properly and also make sure you are hitting all the parts of the question. Before you answer each question, note down the key points you want to hit and evidence you want to use (where applicable). Once you have the skeleton of your response, writing it out will be quick, plus you won’t make any silly mistake in a rush and forget something important.