Bio Chemistry Exam #2 Practice quizzes

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loads DNA Pol delta at sites of single-strand breaks in DNA

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1

loads DNA Pol delta at sites of single-strand breaks in DNA

PCNA

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2

initiates correction of any chemical base damage within DNA (step #1)

repair endonuclease

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3

reacts with G to form N-2 ethylguanosine

acetaldehyde

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4

specifically creates an abasic site at ROS-damaged deoxcytosine within DNA

uracil-DNA glycosylase

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5

converts cytosine to uracil

Superoxide

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6

activates the formation of 5,5,6,6-cyclobutane thymine dimers

260 nm light irradiation

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7

metabolic product of smoke that modifies the 2-amino group of guanosine

Benzo(a)pyrene

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8

strips out the damaged strand which in the case of uv damage activates melanogenesis ("tanning")

Helicase

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9

required to convert uracil to thymine on dUMP

N5,N10-Methylene tetrahydrofolate

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10

Double-stranded DNA is the product of

Replication

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11

Once the polymerase is finished producing DNA, it will only return to the task of template guided synthesis when there is a requirement

Prep for cell division and repair

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12

Fidelity is only ensured by amino acids polymerase active site only during what

Durning Synthesis

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13

Fideltiy is ensured AFTER replication by

detection by repair related proteins

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14

When deletion happens it can happen two ways

Removal of U's by Uracil/DNA glycosylase to create an abasic site

cleavage of a damaged strand by repair endonucleases

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15

Fidelity requires what

Template strand DNA

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16

What does DNA polymerase do after being recruited to site

Repair to replace the damaged strand

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17

with high levels of base damage, repair endonucleases can produce double-strand breaks which hyperactivate

ATM kinase

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18

When ATM kinase is hyperactive what happens

Apoptosis

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19

The substrate it uses is a nucleotide triphosphate (NTP) which it positions to attach to the end of the growing RNA strand using one phosphate to form a

Phospodiester

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20

What is the by product of growing RNA strand

Pyrophosphate

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21

translated by ribosomes into an amino acid sequence- (PROTEIN

mRNA sequencing

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22

A-OH

Amino-acylation

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23

anticodon segment

I G C

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24

patterns of "stem" stacking and loop folding

TRNA

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25

stylized tertiary structure of tRNAs- backbone

Tube worm

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26

decoding the mRNA relies on base-pairing

tRNA anticodons

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27

hypoxanthine can base-pair to the other

Bases

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28

3rd position in the code is called the

Wobble position

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29

amino- acylation is preformed by

aminoacyl-tRNA synthetase

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30

Step 1

aa + ATP => aminoacyl-AMP + PPi

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31

Step 2

aminoacyl-AMP + tRNA =>aminoacyl-tRNA + AMP

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32

this higher energy structure is called an

Acyl phosphate

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33

Iniation

tRNA with mythiene start at AUG

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34

iniation codon

AUG

<p>AUG</p>
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35
<p>Which letter corresponds with pi</p>

Which letter corresponds with pi

E

<p>E</p>
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36
<p>What letter corresponds aminoacyl tRNA</p>

What letter corresponds aminoacyl tRNA

J

<p>J</p>
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37
<p>Which letter corresponds to the tRNA</p>

Which letter corresponds to the tRNA

F

<p>F</p>
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38
<p>Which letter corresponds with the aminoacly #1 carbon is an amide bond</p>

Which letter corresponds with the aminoacly #1 carbon is an amide bond

I

<p><em>I</em></p>
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39
<p>Which letter corresponds with the aminoacyl #1 carbon of the ester bond</p>

Which letter corresponds with the aminoacyl #1 carbon of the ester bond

J

<p>J</p>
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40
<p>Which letter corresponds to the free amino acid</p>

Which letter corresponds to the free amino acid

A

<p>A</p>
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41
<p>Which letter identifies where the amino acyl #1 carbon is in a carboxylate</p>

Which letter identifies where the amino acyl #1 carbon is in a carboxylate

A

<p>A</p>
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42
<p>Which letter corresponds to H2O</p>

Which letter corresponds to H2O

G green

<p>G green</p>
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43
<p>Which letter identifies where the amino acyl #1 carbon is an acyl-phosphate</p>

Which letter identifies where the amino acyl #1 carbon is an acyl-phosphate

H

<p>H</p>
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44
<p>Which letter corresponds to protein</p>

Which letter corresponds to protein

I

<p>I</p>
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45
<p>Which letter corresponds to trigonal bipyramidal geometry</p>

Which letter corresponds to trigonal bipyramidal geometry

C

<p>C</p>
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46
<p>Which letter corresponds with amino acyl AMP</p>

Which letter corresponds with amino acyl AMP

H

<p>H</p>
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47
<p>Which letter corresponds with AMP</p>

Which letter corresponds with AMP

G red

<p>G red</p>
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48
<p>Which letter corresponds with ATP</p>

Which letter corresponds with ATP

B

<p>B</p>
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49
<p>Which letter corresponds to pyrophosphate</p>

Which letter corresponds to pyrophosphate

D

<p><del>D</del></p>
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50

tumor-suppressor protein which is the most studied protein in scientific research

P 53

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51

activates Chk2 kinase

ATM kinase

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52

tag for proteins destined for proteolysis

Ubiquitin

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53

phosphorylates S20 of p53 to block binding by Mdm2

Chk2 kinase

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54

becomes attached in an amide bond between its own C-terminal carboxylate to a lysine on a target protein

Ubiquitin

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55

wobble base of the anticodon of phenylalanine tRNA that base-pairs to ONLY UUU and UUC

Guanine

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56

responds directly to single-strand and strongly to double-strand breaks in DNA

ATM kinase

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57

wobble base of the anticodon of alanine tRNAs that base-pairs to GCX where X is any base

Hypoxanthine

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58

produces "cuts" upstream (5') and downstream (3') on a chemically-damaged DNA strand

Repair endonuclease

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59

directs covalent attachment of ubiquitin to p53

Mdm2

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60

cut by caspase 3 to promote the cutting up of genomic DNA into nucleosome sized fragments, effectively killing the cell

ICDA

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61

trimeric protein that is assembled by p21 to load DNA polymerase delta at sites of repair

PCNA

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62

domain of CBP that bonds to acetyl-lysines to continue the spread of acetylation towards the start of transcription

Bromo

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63

pro-apoptotic protein that is expressed in response to high levels of p53 binding to weaker response element binding sites

Bax

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64

cut by active caspase 9 to promote apoptosis

Pro caspase 3

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65

anti-apoptotic protein which forms inactive heterodimers with Bax to prevent apoptosis

Bcl2

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66

uses phenylalanines to pry open A-T rich, double stranded DNA to expose single-stranded template to RNA Polymerase II

TBP

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67

dimerized by the apoptosome so as to perform cross-proteolytic cleavage

Pro caspase 9

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68

leaked out of the intermembrane space of the mitochondrion to the cytoplasm with cytochrome c to form the apoptosome

Apaf1

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69

inhibited directly by p21 so as to prevent progression through the cell cycle

Start kinase

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70

Contribution of SRP receptor alpha to SRP54 that induces the formation of trigonal bi pyramid intermediate

Arginine finger

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71

As SRP 54 is released the nascent peptide is looped into the opened

Transcolon

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72

The SRP complex stalls translation by preventing the ribosomes interaction with

eEF2

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73

Cuts the polypeptide chain between the pre and the peptide B sections of preproinsulin

Signal peptidase

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74

Bonds with high affinity of the pre portion of preproinsulin to stall translation

SRP54

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75

Acts as a GAP on SRP54 to release it from the nascent peptide

SRP receptor alpha subunit

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76

Small molecule that is bound to SRP54 when it's bound to the nascent peptide

GTP

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77

Cofactor used in redox reactions that produce hydrogen peroxide

FAD

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78

Portion of the nascent peptide that is most important for binding to SRP54

Hydrophobic leucine-rich sequence

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79

ROS produced durning formation of the functional form of pro insulin

Hydrogen peroxide

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80

Enzyme which oxidizes reduced PDI in the rER lumen

Ero1

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81

The most abundant mRNA in the islet beta cell encodes

Preproinsulin

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82

Enzyme that oxidizes the cystines of preproinsulin

Protein disulfide isomerase

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83

organizes the assembly, movement and delivery of transport vesicles moving from the rER to the Golgi

Rab 1

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84

organizes the assembly, movement and delivery of Islet beta cell secretory granules/vesicles from the Golgi to the plasma membrane

Rab 37

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85

motor protein that moves secretory vesicles through the actin cortex to the plasma membrane

myosin5a

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86

cargo receptor carrying BiP, PDI, and Ero1 recognizes what on those proteins to load them into vesicles going from the cis Golgi to the rER

KDEL-COO-

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87

at the delivery point, acts as a GAP on Rabs

HOPS complex

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88

organizes the assembly, movement and delivery of transport vesicles moving from the Golgi to the rER

Rab 2

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89

Cargo receptor that recognizes proinsulin's peptide B/C boundary to load it into secretory granules at the trans Golgi

Prohormone convertase 1

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90

cargo receptor that recognizes proinsulin to load it into vesicles in the rER

Surf4

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91

concentrates protons into the secretory granule in order to mature proinsulin into insulin

V-ATPase

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92

when the secretory vesicle lumen pH is low this cuts the proinsulin molecule between the C and A peptides

Prohormone convertase 2

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93

holds together the B and A peptides in mature insulin

Disulfide bridges

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94

motor that moves vesicles from the rER to the Golgi

kinesin

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95

most directly "snaps" together vesicle and target membranes to effect fusion

snares

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96

motor that moves vesicles from the Golgi to the rER

dynein

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97
term image

Uracil (pyrimidine)

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98
term image

Hypoxanthine (purine)

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99
term image

Cytosine (pyrimidine)

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100
term image

Thymine (pyrimidine)

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