Order Of Draw

Order of Draw

The sequence in which different color-coded tubes are used to collect blood samples during phlebotomy.

Purpose of Order of Draw

Ensures accuracy of blood samples and prevents cross-contamination.

Yellow (Sterile) Tube

Contains SPS (Sodium Polyanethol Sulfonate) and is used for blood cultures.

Light Blue Tube

Contains Sodium Citrate and is used for coagulation studies.

Red Tube

Contains no additive or clot activator and is used for chemistry, serology, and immunology tests.

Gold or Tiger Top Tube

Contains clot activator and gel separator, used for chemistry, serology, and immunology tests.

Light Green Tube

Contains Lithium Heparin and gel separator, used for chemistry tests.

Dark Green Tube

Contains Sodium Heparin and is used for ammonia and HLA typing tests.

Lavender Tube

Contains EDTA (Ethylenediaminetetraacetic Acid) and is used for hematology and blood bank tests.

Gray Tube

Contains Sodium Fluoride and Potassium Oxalate, used for glucose and lactic acid tests.

Royal Blue Tube

Contains no additive or EDTA, used for trace elements and toxicology tests.

Pink Tube

Contains EDTA and is used for blood bank tests.

Light Yellow Tube

Contains ACD (Acid Citrate Dextrose) and is used for DNA and paternity testing.

Importance of Proper Technique

Following the correct order of draw prevents contamination and ensures accurate results for diagnosis and treatment.

1. Blood Culture

2. Light Blue

3. Red

4. Gold/ Tiger

5. Light Green

6. Dark Green

7. Purple/ Lavender

8. Gray

9. Royal Blue

10. Light Yellow

11. Pink

12. Gray

What is the exact order of draw?