Order Of Draw
Purpose of Order of Draw
Ensures accuracy of blood samples
Prevents cross-contamination
Order of Draw Steps
Blood Culture-Yellow Tube
Additive: SPS (Sodium Polyanethol Sulfonate)
Tests: Blood Cultures and Bacterial Studies
Light Blue Tube
Additive: Sodium Citrate
Tests: Coagulation Studies
Must be filled 100%
Red Tube (uncommon)
Additive: “Serum” None or Clot Activator
Tests: Chemistry, Serology, Immunology
Gold or Tiger Top Tube
Additive: Clot Activator and Gel Separator
send out
separates serum and cells
must coagulate 30 mins prior to centrifuge
Tests: Chemistry, Serology, Immunology
Light Green Tube
Additive: Lithium Heparin and Gel Separator
Tests: Chemistry
Dark Green Tube
Additive: Sodium Heparin
Tests: Ammonia, HLA Typing
Lavender Tube
Additive: EDTA (Ethylenediaminetetraacetic Acid)
Tests: Hematology, Blood Bank
Gray Tube
Additive: Sodium Fluoride and Potassium Oxalate
Tests: Glucose, Lactic Acid
Exceptions and Additional Tubes
Royal Blue Tube
Additive: None or EDTA, Sodium Citrate, Heparin, or Serum
Tests: Trace Elements, Toxicology
Pink Tube
Additive: EDTA
Tests: Blood Bank
Light Yellow Tube
Additive: ACD (Acid Citrate Dextrose)
Tests: DNA, Paternity Testing
Importance of Proper Technique
Correct order prevents contamination
Accurate results for diagnosis and treatment
Conclusion
Following the order of draw is crucial for phlebotomy
Ensures accurate and reliable test results
Blood Culture
Light Blue
Red
Gold/ Tiger
Light Green
Dark Green
Purple/ Lavender
Gray
Pink
Light Yellow
Royal Blue
Purpose of Order of Draw
Ensures accuracy of blood samples
Prevents cross-contamination
Order of Draw Steps
Blood Culture-Yellow Tube
Additive: SPS (Sodium Polyanethol Sulfonate)
Tests: Blood Cultures and Bacterial Studies
Light Blue Tube
Additive: Sodium Citrate
Tests: Coagulation Studies
Must be filled 100%
Red Tube (uncommon)
Additive: “Serum” None or Clot Activator
Tests: Chemistry, Serology, Immunology
Gold or Tiger Top Tube
Additive: Clot Activator and Gel Separator
send out
separates serum and cells
must coagulate 30 mins prior to centrifuge
Tests: Chemistry, Serology, Immunology
Light Green Tube
Additive: Lithium Heparin and Gel Separator
Tests: Chemistry
Dark Green Tube
Additive: Sodium Heparin
Tests: Ammonia, HLA Typing
Lavender Tube
Additive: EDTA (Ethylenediaminetetraacetic Acid)
Tests: Hematology, Blood Bank
Gray Tube
Additive: Sodium Fluoride and Potassium Oxalate
Tests: Glucose, Lactic Acid
Exceptions and Additional Tubes
Royal Blue Tube
Additive: None or EDTA, Sodium Citrate, Heparin, or Serum
Tests: Trace Elements, Toxicology
Pink Tube
Additive: EDTA
Tests: Blood Bank
Light Yellow Tube
Additive: ACD (Acid Citrate Dextrose)
Tests: DNA, Paternity Testing
Importance of Proper Technique
Correct order prevents contamination
Accurate results for diagnosis and treatment
Conclusion
Following the order of draw is crucial for phlebotomy
Ensures accurate and reliable test results
Blood Culture
Light Blue
Red
Gold/ Tiger
Light Green
Dark Green
Purple/ Lavender
Gray
Pink
Light Yellow
Royal Blue