Isolation and Enumeration of Bacteriophages

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7 Terms

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noncellular infectious agents that consist of a nucleic acid, covering a protein coat, and sometimes a membranous envelope

viruses

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protein coat

capsid

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bacterial viruses

bacteriophages

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the lysis of bacteria growing confluently on a nutrient agar plate produces clearing on the lawn of bacteria

plaques

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one infectious particle=one visible plaque

PFU

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List the stages of the “life cycle” of a bacteriophage

attachment, penetration, replication, assembly, release

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Formule for calculating PFUs

PFU’s/ml= #plaques x dilution factor x plating factor