BMCB753 Transfer of cell lines, viability determination
0.0(0)
Studied by 0 people
Knowt Play
Learn
Practice Test
Spaced Repetition
Match
FlashcardsCard Sorting
1/8
There's no tags or description
Looks like no tags are added yet.
Study Analytics
Name | Mastery | Learn | Test | Matching | Spaced |
|---|
No study sessions yet.
9 Terms
1
New cards
HBSS
Hanks Balanced Salt Solution
pHed for use in air
2
New cards
HBSS modified
No calcium or magnesium
Used to rinse cells in passaging
3
New cards
Complete HBSS
Complete salt in terms of types and quantities of ions
4
New cards
What to look for when trypsinizing
rounded, loosely attached, floating cells
sheets of cells lifting from flask
5
New cards
Log phase for karyotyping/mitotic index
Cells are still dividing (metaphase)
Get an accurate count for mitotic
Enough dividing cells to count chromosomes
6
New cards
100% confluence of T25 CHO
5-6 million
7
New cards
100% confluence of T25 SKOV3
2-3 million
8
New cards
CHO doubling time
24 hours
9
New cards
SKOV3 Doubling time
33 hours
Note 
Flashcards (21)