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1. A written procedural protocol should contain the following information, in the correct order: ____, ____, ____, ____. Choose from a to d in the answer list. a. Specimen collection and storage b. Reference values c. Reagents, supplies, and equipment d. Procedural method a. A, B, C, D b. B, C, A, D c. A, C, D, B d. D, C, B, A
C
2. Factors that can denature, coagulate, or alter protein molecules include: a Heat b. Strong acid solution c. Strong alkali solution d. All of the above
D
3. If testing cannot be done within _____ hours of collection, a serum specimen should be frozen at −20°C. a. 24 b. 48 c. 72 d. 96
C
4. Complement can be inactivated in human serum by heating to _____ °C. a. 25 b. 37 c. 45 d. 56
D
5. A specimen should be reinactivated when more than ______ hour(s) has (have) elapsed since inactivation. a. 1 b. 2 c. 4 d. 8
C
6. A graduated pipette can be used when: a. Extreme accuracy is not needed b. Very precise accuracy is needed c. Precision is more important than speed d. Precision and speed are important
A
7. A meniscus is the: a. Curvature in the top surface of a liquid b. Zero mark on a pipette c. Last marking on a serologic pipette d. Flat line of liquid in a pipette
A
8. Automatic pipettes have the advantage of: a. Being fast b. Allowing repetitive measurement of solutions c. Delivering equal volumes of solutions d. All of the above
D
9. A dilution is a(n): a. Ratio of volume or number of parts of the substance to be diluted in the total volume, or parts, of the final solution b. Indication of relative concentration c. Frequently used measure in serologic testing d. All of the above
D
10. If a serial dilution is prepared in 1:2 dilutions, the final dilution in tube 6 is: a. 1:25 b. 1:32 c. 1:64 d. 1:256
C
11. To prepare 10 mL of a diluted serum specimen 1:10, ____ part of serum is needed: a. 1.0 b. 0.75 c. 0.50 d. 0.20
A
12. To prepare 10 mL of a diluted serum specimen 1:10, ____ part(s) of distilled water is (are) needed to reach the total volume: a. 10 b. 9 c. 4.5 d. 0.1
B
13. Serum for detection of antibodies should be drawn during the: a. Acute phase of illness only b. Acute and convalescent phases of illness c. Convalescent phase of illness only d. Acute and convalescent phases and also 6 months after an illness
B
14. A central concept of serologic testing is: a. Antigen-antibody interaction b. Determination of antibody composition c. Quantitation of antigen titer d. Manifestation of a rise in antibody titer
D
15. A major advantage of rapid POCT testing is: a. Faster turnaround time b. Lower cost c. Better quality than traditional testing d. Both a and c
A
16. Rapid testing assays are usually in the ____CLIA category: a. Waived b. Provider-performed microscopy c. Moderately complex d. Highly complex
A
17. Over-the-counter test kits are in the ________ CLIA category: a. Waived b. Provider-performed microscopy c. Moderately complex d. Highly complex
A
18. Rapid turnaround time _____ an important characteristic of POCT. a. Is b. Is not
A
19. Ease of performance _____ an important characteristic of POCT. a. Is b. Is not
A
20. Storage temperature of reagents _____ an important characteristic of POCT. a. Is b. Is not
A
21. Length of time until reagent expiration _____ an important characteristic of POCT. a. Is b. Is not
A