7 - QUANTITATIVE CLOT RETRACTION

3

1, 37, room temperature

STEFANINI METHOD (TEST TUBE METHOD)

1. Draw _ mL fresh venous blood

2. Transfer blood into a test tube

3. Incubate for _ hour (at _ C), after which let the tube stand at _ _ and observe for retraction to take place

appearance

STEFANINI METHOD (TEST TUBE METHOD)

Results

Grading: According to the _ of the clot

Complete

Partial

Poor

Very Poor

STEFANINI METHOD (TEST TUBE METHOD)

Results

Grading (4)

5

applicator stick

MACFARLANE METHOD

1. Collect _ mL blood. Place the blood into a clean calibrated tube/plain test tube

2. Place an _ _ inside the tube and cover it using a cork with a bore/hole at the center

Applicator stick

MACFARLANE METHOD

Is where the clot will attach into

37, 5-10

1, room temperature, retraction

MACFARLANE METHOD

1. Collect 5 mL blood. Place the blood into a clean calibrated tube/plain test tube

2. Place an applicator stick inside the tube and cover it using a cork with a bore/hole at the center

   • Applicator stick: Is where the clot will attach into

3. Place the tube in _ C water bath and observe every _-_ minutes for coagulation

4. _ hour after firm clotting, allow tube to stand just at _ _ for retraction to take place (_ has taken place if the clot has shrunk and has attached itself onto the applicator stick)

Serum

volume of expressed serum, calibration

MACFARLANE METHOD

1. Collect 5 mL blood. Place the blood into a clean calibrated tube/plain test tube

2. Place an applicator stick inside the tube and cover it using a cork with a bore/hole at the center

   • Applicator stick: Is where the clot will attach into

3. Place the tube in 37 C water bath and observe every 5-10 minutes for coagulation

4. One hour after firm clotting, allow tube to stand just at room temperature for retraction to take place (retraction has taken place if the clot has shrunk and has attached itself onto the applicator stick)

5. After retraction has taken place, remove the cork to remove the applicator stick and the clot

   • _ will be left in the tube

6. Measure the _ _ _ _ directly against the _ of the tube (if using plain test tube, measure the volume of serum using a graduated cylinder)

7. Compute for clot retraction as follows

44-67%

MACFARLANE METHOD

Normal Value

% CR = amount of serum expressed/amount of whole blood used x 100

MACFARLANE METHOD

Formula