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Transcription
The information contained in the DNA is copied into a complementary strand of RNA (ribonucleic acid)
This RNA copy is called
mRNA
RNA processing
the mRNA copy needs to be modified (cleaned-up) before it leaves the nucleus - Does not occur in prokaryotic cells
Translation
Ribosomes read the mRNA and make a protein
Constitutive genes
Some genes are transcribed continuously independent of cell health or environmental conditions
Example: ATP synthase, actin, tubulin
Regulated expression
Allows for much tighter control of gene expression - Signal from outside or inside the cell starts the whole process
All genes (eukaryotic and prokaryotic) have a unique region of DNA sequence upstream of (before) the transcriptional start site that serves as a binding site for the ____________(makes RNA)
RNA polymerase
Promoter Recognition
Tells the cell where the gene of interest is
This is critical – transcribing the wrong gene could be deadly
- Each gene has a unique promoter sequence
Tell RNA polymerase
Analogy: Front cover of a book