Troubleshooting Special Stains for Connective Tissues, Muscle Fibers, and Lipids 낱말 카드 | Quizlet

What can cause poor Jones' methenamine silver staining?

thick sections

What can cause granular background deposits in Jones' methenamine silver staining?

metal fixatives used

What can cause heavy background staining in Jones' methenamine silver staining?

over exposure to methenamine silver solution

What can cause uneven staining in Jones' methenamine silver staining?

silver impregnation is stopped early

What can cause decreased "red" staining in Masson's trichrome?

old Biebrich scarlet acid-fuchsin solution or overused solution

What can cause faded "blue" staining in Masson's trichrome?

over differentiation in acetic acid solution

What can cause weak or no staining in Oil red O (ORO) staining?

improper fixation or processing with non-frozen sections

What can cause weak staining in Reticulum staining?

improperly prepared ammoniacal silver solution, insufficient exposure time, incorrect dilution of formalin

What causes weak staining of elastic fibers in Verhoff-Van Gieson (VVG) staining?

over differentiation, poor counterstain timing, varying elastic fiber content

What maximizes the shelf-life of Jones' methenamine silver?

refrigerate it, or make fresh before use

What causes a heavy black precipitant in Jones' methenamine silver solution or Reticulum staining?

exposure to metal