More gentics vocab 3

cell or individual with some deviation from a whole multiple of the haploid or monoploid chromosome complement (eg. having one extra chromosome or missing one chromosome) is most correctly called

aneuploid.

The procedure whereby RNA is separated by agarose gel electrophoresis, transferred to membrane and incubated with a specific probe is often referred to as

Northern blotting.

The general approach of moving from a cloned gene to identifying a mutation in that gene is called

reverse genetics.

The situation where no DNA is present in excess or missing from the chromosomal complement is referred to as

balanced.

The ability of some phage to transfer any appropriately sized segment of a bacterial chromosome between bacteria is called

generalized transduction.

Some commonly grown garden plants have four copies of each chromosome, all derived from the same original species. This is best characterized as an example of

Autopolyploidy.

A chromosomal rearrangement in which the two long arms of two acrocentric chromosomes fused to create a new chromosome is called a

Robertsonian translocation

RNA-mediated interference (RNAi)

A method whereby introduction of double-stranded RNA into cells leads to degradation of the corresponding endogenous gene product, producing a mutant-like phenotype.

endosymbiont

an organism, usually prokaryotic, residing within the cytoplasm of a host cell

homoplasmy

having mitochondria that are all wild type or all mutant.

A method whereby introduction of double-stranded RNA into cells leads to degradation of the corresponding endogenous gene product, producing a mutant-like phenotype, is called

RNA-mediated interference.

A bacterial anti-viral defense mechanism that has been adapted to create or correct mutations within cells is called

CRISPR/Cas9.

Some commonly grown garden plants have four copies of each chromosome, all derived from the same original species. This is best characterized as an example of

Autopolyploidy.

A cell or individual with some deviation from a whole multiple of the haploid or monoploid chromosome complement (eg. having one extra chromosome or missing one chromosome) is most correctly called

aneuploid.

A chromosomal rearrangement in which non-homologous chromosomes swap corresponding segments of DNA is called

reciprocal translocation.

The procedure whereby RNA is separated by agarose gel electrophoresis, transferred to membrane and incubated with a specific probe is often referred to as

Northern blotting.

An organism with only one copy of each chromosome is said to be

monoploid.

heteroplasmy

having a mixture of mutant and wild type mitochondria

endosymbiont

an organism, usually prokaryotic, residing within the cytoplasm of a host cell

transduction

the transfer of DNA from one bacterial cell to another by a virus.

maternal effect

the mother’s genotype determines the offspring’s phenotype through factors deposited in the egg either as RNA or protein

cloning yourself

Obtain an egg from a specied
Remove its nucleus
Fuse the enucleated egg to a somatic cell from what you want to clone
Implant the embryo into a host female to permit development to term.

antibody probe

You would screen a cDNA library in an expression vector by colony lift using the antibody as probe

reposter transgene

You would produce transgenic mice that contain a reporter transgene that fuses mgc promoter

mouse model

1. Infect ES cells from black mouse using vector shown above. 2. Select for recombinants in neomycin and ganciclovir. 3. Verify that recombinants had deletion within mgc by Southern or PCR. 4. Mix engineered ES cells with embryo derived from white mouse. 5. Implant into pseudopregnant mouse to produce chimeric mice. 6. Cross chimeric X white to find those chimeric mice that produce all black offspring. Use Southern or PCR to ID
hets. 7. Cross hets to generate homozygotes

Extranuclear inheritance

The trait is determined by mutation of DNA that resides outside the nucleus (in this case presumably within mitochondria).

Functional complementation

ibrary from wild type, transform mutant, find rescue