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cellular respiration
the oxidation of glucose (and other molecules), and the capture of energy in a form that is useful to the cell and the cell’s source of ATP energy
net reaction of cellular respiration
C6H12O6 + 6O2 –> 6CO2 + 6H2O + energy (ATP)
glycolysis
occurs in the cytosol, does not require oxygen, also occurs in bacterial cells, and it dates from the earliest days of life
cytosol
where glycolysis occurs
NADH
passes their electrons to the electron transport chain in the inner mitochondrial membrane
citric acid cycle
describe later
oxidative phosphorylation
the result of this is most of the ATP yield from cellular respiration
reduced electron carriers
NADH and FADH2
electron transport
reduced NADH and FADH2 (from the oxidation of food molecules) pass their electrons to the electron transport chain in the inner mitochondrial membrane
inner mitochondrial membrane electron carriers
the electrons lose energy as they move from electron carrier to electron carrier in the membrane
H+ pumping across the inner membrane
energy is used to pump H+ ions, against their
electrochemical gradient into intermembrane space
intermembrane space
the space between the inner and outer membrane of the mitochondrion
proton gradient
ATP synthase
The potential energy stored in the form of the H+ gradient is used by this to make ATP
O2 evolution
electron transport blockers
poisons can block transport as well as suffocation
uncouplers
allow electron transport to continue but dissipate the H+ gradient
fermentation
happens when there is no oxygen available
lactate
during fermentation pyruvate from glycolysis is converted to lactate or ethanol instead of acetyl-CoA
function of fermentation
it allows the NADH that is formed in glycolysis to dump its electrons somewhere and cycle back to glycolysis as NAD+, thus keeping glycolysis and its ATP production going
other food molecules
molecules other than glucose (other sugars, amino acids, fragments of fatty acids) can be fed into the pathway at various points, so they can supply metabolic energy too
intermediates
drawn off from the pathway to serve as precursors in biosynthetic reactions
biosynthetic reactions
used to construct the organic molecules of the cell
light reactions
energy from light is used to create temporary chemical energy in the form of ATP and high-energy electrons carried by NADPH
Calvin cycle
temporary chemical energy from the light reactions is used to create stable, storable chemical energy – carbohydrate
CO2 fixation
the incorporation of carbon from CO2 into organic compounds entering the Calvin Cycle
photosynthetic pigments
chlorophylls and carotenoids
chlorophyll a
participates directly in the light reactions and absorbs mainly blue-violet and red light
chlorophyll b
absorbs mainly blue and orange light and broadens the range of light a plant can use
carotenoids
various shades and yellow and orange and gives pigment to foods like carrots
absorption spectrum
color light they absorb during photosynthesis
photosystem
an assembly of several light harvesting complexes that gather sunlight and pass the energy to a reaction center complex
pigments of photosynthesis
pigments in the light-harvesting complex absorb light and transfer the energy to the reaction center chlorophyll a
reaction center chlorophyll a
chlorophyll a molecules are oxidized after absorbing the energy from the light-harvesting complex
chlorophyll oxidation
primary electron acceptor
an excited electron is captured by the acceptor
photophosphorylation
The production of ATP by chemiosmosis during the light reactions of photosynthesis
Calvin cycle
CO2 reduction (CO2 fixation)
the Calvin cycle makes carbohydrate by fixing (reducing) CO2
14^C tracer
in the 1940s, Melvin Calvin discovered using radioactive carbon (14C) as a tracer
rubisco
the first enzyme of the Calvin cycle
RuBP
the first reaction of the Calvin cycle
3-phosphoglycerate
RuBP (5C) + CO2 (1C) –> 2x3-phosphoglycerate (2x3C)
1-glyceraldehyde-3-phosphate
net gain of the Calvin cycle
3 turns (that is, 3 carbons fed in) –> 1 glyceraldehyde-3-phosphate