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Anton van Leeuwenhoek
Developed the first microscope
Observed pond water and discovered the microscopic life that lives within
Robert Hooke
First person to use the term “cells” by observing plant cell walls in cork
Mitosis
Regular cell division
Most cells replicate by
making exact copies of themselves
Robert Brown
Discovered that many cells have a dark spot near the center
Credited with discovering the nucleus
Matthias Schledien
Stated that “all plants are made of cells”
Theodor Schwann
Stated that “all animals are made of cells”
Rudolf Virchow
stated that “all cells arise from the division of preexisting cells”
Transmission Electron Microscope magnification
capable of over 1,000,000x magnification
What must be done to specimens to be placed in the Transmission Electron Microscope?
Frozen or embedded in plastic and sliced thin
How do Transmission Electron Microscopes work?
Use magnets to focus a beam of electrons on the specimen.
Scanning electron microscope magnification
100,000x
Scanning electron microscope is capable of
producing a 3-D, photograph-type image
How does the scanning electron microscope work?
beam of electrons sweeps across the object and bounces back to be interpreted by a computer
Neither the TEM or the SEM use
Lenses
A magnifying glass is a
Simple light microscope
The word “light’ in a light microscope means…
you actually see the light reflecting off the surface of the specimen
In a compound light microscope, the word compound means…
it has two lenses (objective and ocular/eyepiece)
To calculate the total magnification in the compound light microscope, you…
multiply the objective lens magnification times the eyepiece magnification
Scanning objective lens
Red, 4x
Low power objective lens
Yellow, 10x
High Power objective lens
Blue, 40x
What is #1
Ocular
What is #2
Eye piece
What is #3
Revolving nosepiece
What is #4
Objective lenses
What is #5
Diaphragm
What is #6
Stage
What is #7
Light source
What is #8
Base
What is #9
Coarse adjustment
What is #10
Fine adjustment
What is #11
Arm
What is #12
Stage clips
Resolution
ability to distinguish between two objects
What does the diaphragm do?
Adjusts the level of light
What does the stage do?
Specimen is placed on the stage
What does the revolving nosepiece do?
Allows objective lenses to be rotated
What do the coarse and fine adjustments do?
Raise and lower the stage
When you pick up a microscope, you should have…
one hand supports the arm, one hand supports the base
Cell theory #1
All living things are made up of one or more cell.
Cell theory #2
The cell is the basic unit of organization of living things.
Cell theory #3
All cells come from pre-existing cells.