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What does R represent in tRNA notation?
Purines
What does Y represent?
Pyrimidines
What does y represent?
Pseudouridine
Why is pseudouridine important?
Most abundant RNA modification
Which enzymes charge tRNAs?
Aminoacyl
What is the first step of tRNA charging?
Amino acid activation
What intermediate is formed?
Aminoacyl
What chemical attack forms aminoacyl
AMP?
What is the second step of charging?
Transfer to tRNA 3′ end
Which nucleotide attacks the adenylate?
Adenosine 76
What is the final product?
Aminoacyl