6_Characterization of Extracted Plant Pigments

solvent front

The mark where the solvent stopped is called the __

Rf (Reference front)

This migration of pigment relative to migration of solvent is expressed as a constant ___

= distance pigment migrated / distance solvent front migrated

Rf value can be calculated by using the formula:

Paper chromatography

Is a technique that can separate compounds on paper by a solvent system that carries the mixture of pigments up the paper by capillary action

the mobile phase and stationary

In paper chromatography, two phases are formed:

solvent

In paper chromatography, the mobile phase focuses on the:

chromatography paper

In paper chromatography, the stationary phase focuses on the:

difference in distribution of the components

In paper chromatography, the separation is caused by a ___ between the tationary phase and the mobile phase

absorbance spectrum

• Plant pigments have specific wavelength absorbance patterns known as the ____

• this will help determine which light waves are absorbed by the leaf pigments

Eluents

After cutting the bands from strips, grouping according to color, putting in a tube containing 4 ml of pure acetone, and shaking it for 5-10 mins, the partially purified pigments are called ___

blue-green

Chlorophyll a is color

Yellow-green

Chlorophyll b is color

Orange

Carotene is color

Yellow

Xanthophyll is color

UV/Vis Spectrophotometry

a technique utilized in measuring how much radiant energy a substance absorbs at various wavelengths.

visible spectra (380 nm to 760)

Pigments and colored materials normally absorb in the range of the ___

tungsten lamp

Spectrophotometers that operate for absorption measurements between 340 to 1000 nm use a ____as a light source

Spectrophotometer

The ability of a pigment to absorb various wavelengths can be measured with a____

chlorophyll

• the primary photosynthetic pigment

• absorbs light most strongly in the red and blue regions of the spectrum but reflects much of the green light

red-purple, blue-black

Anthocyanin is color ___

Yellow-orange

Zeaxanthin is color___

red

Phycoerythrin is color___