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What is ATP composed of?
ribose (a sugar), adenine (a nitrogenous base), and three phosphate groups.
How is energy released from ATP?
when the terminal phosphate bond is broken by hydrolysis.
What creates potential energy in ATP?
Repulsion between the negative charges of the three phosphate groups creates a lot of potential energy, likened to a compressed spring.
What types of cellular work are powered by ATP hydrolysis?
mechanical, transport, and chemical processes
What is phosphorylation in the context of ATP?
the transfer of a phosphate group, typically used to power endergonic reactions.
What is a phosphorylated intermediate?
a recipient molecule that is more reactive (less stable, with more free energy) than the original molecule.
What effect does ATP hydrolysis have on proteins?
causes a change in protein shape and binding ability.
How is ATP regenerated?
by the addition of a phosphate group to adenosine diphosphate (ADP).
Where does the free energy needed to phosphorylate ADP come from?
exergonic breakdown reactions (catabolism).
What is the ATP cycle?
involves the shuttling of inorganic phosphate and energy, coupling energy-yielding processes to energy-consuming ones.
Metabolism
totality of an organism's chemical reactions
Metabolic pathway
In this, a specific molecule is altered in a series of steps to produce a product.
Catabolic pathways
release energy by breaking down complex molecules into simpler compounds.
Anabolic pathways
consume energy to build complex molecules from simpler ones.
Downhill reactions
Catabolic pathways are described as this.
Uphill reactions
Anabolic pathways are described as this
Bioenergetics
the study of how energy flows through living organisms.
Energy
the capacity to cause change, can be used to do work—move matter against opposing forces, such as gravity and friction.
Kinetic energy
energy associated with motion.
Thermal energy
kinetic energy associated with random movement of atoms or molecules.
Heat
Thermal energy in transfer from one object to another
Potential energy
energy that matter possesses because of its location or structure.
Chemical energy
potential energy available for release in a chemical reaction.
Example of chemical energy
Complex molecules, such as glucose, are high in chemical energy because energy is released as they are broken down to simpler products.
Thermodynamics
the study of energy transformations in a collection of matter.
System
In thermodynamics, refers to the matter being studied.
Surroundings
In thermodynamics, refers to the universe.
Isolated system
cannot exchange energy or matter with its surroundings.
Closed system
heat can transfer but energy cannot.
Open system
can exchange energy and matter with its surroundings.
First law of thermodynamics
Energy can be transferred and transformed, but it cannot be created or destroyed.
Energy of the universe
constant
Second Law of Thermodynamics
Every energy transfer or transformation increases the entropy of the universe.
Entropy
A measure of molecular disorder, or randomness.
Spontaneous Processes
Processes that occur without energy input; they can happen quickly or slowly.
Nonspontaneous Processes
Processes that decrease entropy and require an input of energy.
Gibbs Free Energy (G)
The portion of a system's energy that can do work when temperature and pressure are uniform throughout the system.
Change in Free Energy (ΔG)
Indicates whether a reaction is spontaneous or not.
Exergonic Reaction
A chemical reaction that proceeds with a net release of free energy to the surroundings.
Endergonic Reaction
A chemical reaction that absorbs free energy from the surroundings.
Equilibrium
The point at which forward and reverse reactions occur at the same rate, describing a state of maximum stability.
Free Energy Change Equation
ΔG = ΔH - TΔS, where ΔH is the change in enthalpy, T is temperature in Kelvin, and ΔS is the change in entropy.
Free Energy and Stability
Free energy can be thought of as a measure of a system's stability; unstable systems (higher G) tend to become more stable (lower G).
Energy Flow in Ecosystems
Energy flows into ecosystems as light and exists as heat.
Spontaneous Process and Work
A process is spontaneous and can perform work only when it is moving toward equilibrium.
Thermal Energy Loss
For every energy transfer or transformation, some energy is converted to thermal energy and lost as heat.
Processes Increasing Entropy
Can occur spontaneously.
Processes Decreasing Entropy
Are nonspontaneous and require an input of energy.
Endomembrane System
consists of the nuclear envelope, endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, and plasma membrane, which are either continuous or connected via transfer by vesicles.
Endoplasmic Reticulum (ER)
accounts for more than half of the total membrane in many eukaryotic cells and is continuous with the nuclear envelope.
Smooth ER
synthesizes lipids (including oils, phospholipids, & steroids), metabolizes carbohydrates, detoxifies drugs and poisons, and stores calcium ions.
Rough ER
has bound ribosomes, which secrete glycoproteins and distributes transport vesicles, serving as a membrane factory for all-bound proteins synthesized in it.
Golgi Apparatus
consists of flattened membranous sacs called cisternae, modifies products of ER, manufactures macromolecules, and sorts and packages materials for transport.
Lysosomes
membrane-bound sacs of hydrolytic enzymes that an animal cell uses to digest macromolecules, working best at acidic pH.
Vacuoles
large vesicles derived from the ER and Golgi apparatus that perform a variety of functions, including food vacuoles formed by phagocytosis.
Contractile Vacuoles
found in many freshwater protists, pump excess water out of cells.
Central Vacuoles
found in many mature plant cells, contain a solution called sap and play a major role in plant growth.
Mitochondria
the sites of cellular respiration, the metabolic process that uses oxygen to generate ATP, and are found in nearly all eukaryotic cells.
Chloroplasts
found in plants and algae, the sites of photosynthesis and contain the green pigment chlorophyll as well as enzymes for sugar production.
Chloroplast
One of a group of plant organelles called plastids.
Thylakoids
Membranous sacs stacked to form granum within chloroplasts.
Stroma
The internal fluid of chloroplasts containing DNA, ribosomes, and enzymes.
Plastids
A group of plant organelles, including chloroplasts and others.
Endosymbiont Theory
Suggests that an early ancestor of eukaryotes engulfed a non-photosynthetic prokaryotic cell, leading to the evolution of mitochondria and chloroplasts.
Peroxisomes
Specialized metabolic compartments bounded by a single membrane that contain enzymes to remove hydrogen atoms and form hydrogen peroxide (H2O2).
Compartmentalization
The separation of enzymes that produce hydrogen peroxide and those that dispose of it to prevent damage to cellular components.
Cytoskeleton
A network of fibers extending throughout the cytoplasm that organizes the cell's structures and activities.
Microtubules
Cytoskeletal structures that shape the cell, guide movement of organelles, and separate chromosomes during cell division.
Microfilaments
A network that helps support the cell's shape and forms a cortex just inside the plasma membrane.
Intermediate filaments
More permanent cytoskeleton fixtures that support cell shape and fix organelles in place.
Cell Wall
An extracellular structure that distinguishes plant cells from animal cells, providing protection and maintaining shape.
Primary cell wall
The relatively thin and flexible wall secreted first in plant cells.
Middle lamella
A thin layer between primary walls containing polysaccharides called pectins.
Secondary cell wall
An additional layer added between the plasma membrane and the primary cell wall in some cells.
Glyoxysomes
Peroxisomes in seeds that convert fatty acids to sugar to feed seedlings.
ATP
A molecule that serves as the energy currency of the cell, produced by mitochondria.