BACT211 Midterms: Lesson 5 - Vibrio, Aeromonas, Campylobacter, and Helicobacter (Part 2)

Most clinically relevant species of Campylobacter

1. C. jejuni subsp. jejuni

2. C. coli

3. C. fetus subsp. fetus

A disease associated with Campylobacter, which manifests as rapid onset muscle weakness caused by the immune system, damaging the Peripheral Nervous System (PNS).

Guillain-Barré Syndrome

most common cause of bacterial gastroenteritis worldwide

Campylobacter jejuni

MOT of Campylobacter

1. Direct contact with animals and handling infected pets, such as dogs, cats, and birds.

2. Indirectly by the consumption of contaminated water and dairy products and improperly cooked poultry

3. Person-person transmission

Campylobacter species, which is the causative agent of bacteremia

Campylobacter fetus

a fatty acid binding protein seen in PNS, myelin and human astrocytes, the star-shaped glial cells of the CNS, which is isolate from blood cultures infected with C. fetus.

PERIPHERAL MYELIN PROTEIN 2 (P2)

Specimen of Choice for Campylobacter jejuni

Stool

Transport media used for C. jejuni if there is delay in processing

Cary-Blair

Specimen of choice for C. fetus

blood with incubation at 35°C to 37°C

Selective media used for C. jejuni

Campy-BAP

Other selective media that can be used for C. jejuni

1. Butzler Medium

2. Skirrow’s Mediu

Disease associated with H. pylori

TYPE B Gastritis or Peptic Ulcer

A bacteria which is considered carcinogenic

Helicobacter pylori

Specific cancer associated with long term H. pylori infection

1. Gastric adenocarcinoma

2. Gastric MALT lymphoma

Colonizes the stomach for a long time and can cause a low-grade inflammatory process

Helicobacter pylori

Specimen of Choice for Helicobacter

gastric biopsy material

Transport Medium used for Helicobacter

Stuart Medium

Selective used for Helicobacter

Skirrow’s Agar

Incubation temperature for C. jejuni

42° C

Incubation temperature for H. pylori

37°C

urved non-spore-forming, gram-negative rods, which may appear as long spirals or 'S' or seagull wing shapes

Campylobacter spp.

Recommended counter stain in staining Campylobacter spp.

Carbol fuschin

Motility possessed by Campylobacter spp. on hanging drop preparations

"Darting" motility (corkscrew movement)

Determine whether Campylobacter or Helicobacter spp.

SINGLE polar flagellum

Campylobacterr spp.

Determine whether Campylobacter or Helicobacter spp.

MOTILE with MULTIPLE flagella at one pole

Helicobacter spp.

What are the results of Campylobacter and Helicobacter on Oxidase

Both oxidase positive

What are the results of C. jejuni and H. pylori in Urease

C. jejuni - (-)

H. pylori (+)

What are the results of C. jejuni and H. pylori in Hippurate Hydrolysis Tests

H. pylori (-)

C. jejuni (+)