Biochemical Reactions
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85 Terms
Triple Sugar Iron Agar
Identify the medium.
Triple Sugar Iron (TSI) Reaction
This test differentiate organisms producing cysteinase enzyme from those organisms that do not produce cysteinase
cysteinase
This test differentiate organisms producing ______ enzyme from those organisms that do not produce _____.
butt portion
Which part of this medium is the anaerobic portion?
slant portion
Which part of this medium is the aerobic portion?
0.1% glucose
Composition of the butt portion of this medium.
1% lactose and 1% sucrose
Composition of the slant portion of this medium.
Phenol red
pH indicators in this medium
Ferrous sulfate and Sodium thiosulfate
H2S indicators in this medium
lactose and hydrogen sulfide indicators
What is responsible for the differential property of this medium?
lactose, ferrous sulfate and sodium thiosulfate
What is content/s is/are responsible for the differential property of this medium?
Sugar fermentation, H2S production, Gas production
Give the 3 Reactions involved in this medium.
Cysteinase
What enzyme is responsible for the black precipitate present in Test Tube B?
Yellow slant over yellow butt, with gas, without hydrogen sulfide
Interpret the reaction of Test Tube A
Acid slant over acid butt, with gas, without hydrogen sulfide
Define the reaction of Test Tube A
Glucose is fermented, lactose and/or sucrose are fermented
Interpret the result of Test Tube A
Red slant over yellow butt, with gas, with hydrogen sulfide
Interpret the reaction of Test Tube B
Alkaline slant over acid butt, with gas, with hydrogen sulfide
Define the reaction of Test Tube B
Glucose is fermented, lactose and/or sucrose are not fermented
Interpret the result of Test Tube B
Indole Production Test
What biochemical test is involved in this set-up?
Indole Production Test
What biochemical test is used to test the ability of the organism to produce the enzyme tryptophanase?
tryptophanase
This biochemical test is used to test the ability of the organism to produce the enzyme _______?
tryptophanase
What enzyme destroys tryptophan, producing indole?
Kovac’s reagent (paradimethylaminobenzaldehyde)
Indole is demonstrated by adding what reagent?
Indole
What is demonstrated in this set-up when Kovac’s reagent is added?
Tryptone Broth
Identify the medium.
Methyl Red Test
What biochemical test is involved in this set-up?
Methyl Red - Voges Proskauer Broth
Identify the medium.
Methyl Red Test
What biochemical test is used to detect the presence of acid products in the medium that the organism produces from sugar fermentation?
Methyl red indicator
What reagent is used in this set-up? This is added to detect the acids in the culture medium.
Voges-Proskauer Test
What biochemical test is involved in this set-up?
Methyl Red - Voges Proskauer Broth
Identify the medium.
Voges-Proskauer Test
What biochemical test is used to detect acetoin and butanediol as the energy source for the organisms in the culture medium?
acetoin and butanediol
What is/are detected in this set-up which serves as the energy reserve of the organisms in the culture medium?
Potassium hydroxide
alpha-naphthol
What reagent is used in this set-up?
Alkali-sensitive and Oxygen-dependent
What are the 2 requirements in this set-up?
Citrate Utilization Test
What biochemical test is involved in this set-up?
Citrate Utilization Test
What biochemical test detects the ability of the enteric organism to utilize the citrate as the carbon source?
Sodium citrate
What is the only carbon source in this medium?
Simmons Citrate Agar
Identify the medium.
Bromthymol blue
What is the pH indicator in this medium?
Reduction of Nitrates to Nitrites
What biochemical test is involved in this set-up?
Reduction of Nitrates to Nitrites
What biochemical test detects the ability of the organism to produce the enzyme nitrate reductase?
Nitrate reductase
What enzyme is detected in this set-up?
Nitrate Broth
What culture medium is used in Reduction of Nitrates to Nitrites?
Sulfanic acid
alpha-naphthylamine
What reagent/s is/are used in this set-up?
Sulfite Indole Motility (SIM) Test
What biochemical test is involved in this set-up?
Sulfite and Indole
What 2 other subreactions are detected in this set-up?
Sulfite Indole Motility Medium
Identify the medium.
Ferrous sulfate and Sodium thiosulfate
H2S indicators in this medium
Motility, H2S production, and Indole production
What are the 3 reactions detected in this medium?
Urease Test
What biochemical test is involved in this set-up?
Urease Test
What biochemical test detects the ability of the organism to produce the enzyme urease?
Urease
What enzyme is detected in this medium?
Proteus organisms
This test is a rapid identification test for what organisms?
Urease Test
This biochemical test is a rapid identification for Proteus organisms.
Christensen’s Urea Broth
Identify the medium.
Phenol red
pH indicator in this medium
Ammonia
What product of urease will alkalanize the entire broth in this set-up?
Phenylalanine Deamination Test
What biochemical test detects if the organism produces the enzyme phenylalanine deaminase?
Phenylalanine deaminase
What enzyme is being detected in Phenylalanine Deamination Test?
Phenylalanine Deamination Test
What biochemical test identifies Proteus group of organisms?
phenyl pyruvic acid
Deamination of phenylalanine yields what?
Carbohydrate Fermentation Test
What biochemical test is involved in this set-up?
Sugar Fermentation Test
Carbohydrate Fermentation Test is also known as?
Cysteine Trypticase Agar
Identify the medium.
Phenol red
pH indicator in this medium
Lysine Decarboxylation and Deamination Test
What biochemical test is involved in this set-up?
Lysine Decarboxylation and Deamination Test
What biochemical test detects the ability of the organism to produce the lysine decarboxylase and lysine deaminase?
Lysine decarboxylase and Lysine deaminase
What are detected in this set-up?
Lysine Iron Agar
Identify the medium.
Glucose
What sugar is present in this medium?
Lysine
What is the amino acid content which is also the differential property in this medium?
Bromcresol purple
pH indicator in this medium
Ferrous ammonium and Sodium thiosulfate
H2S indicators in this medium
Glucose fermentation, Lysine deamination, Lysine decarboxylation, and H2S production
What are the 4 reactions detected in this medium?
Ornithine Decarboxylation Test
What biochemical test is involved in this set-up?
Ornithine Decarboxylation Test
This biochemical test detects the ability of the organism to produce the ornithine decarboxylase.
Ornithine Decarboxylation Test
This single biochemical test differentiates the Proteus species.
Ornithine decarboxylase
What is being detected in this set-up?
Motility Indole Ornithine medium
Identify the medium.
Glucose
Sugar present in this medium
Ornithine
Amino acid content in this medium
Bromcresol purple
pH indicator in this medium
Glucose fermentation, Ornithine decarboxylation, motility, and indole production
What are the 4 reactions being detected in this medium?
Note 
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