What is the difference between G-actin and F-actin?
G-actin is GLOBULAR, F-actin is FILAMENT
List a few actin-binding proteins:
-ARP2/3 complex
-tropomodulin
-myosin V
-Catenin and Vinculin
What is treadmilling?
When there is actin de-polarization and polymerization occuring at the same time.
Why is targetting actin not possible for cancer drugs?
Too Toxic to the cells, not specific to actin in cancers
What is a tubulin dimer composed of?
1 alpha tubulin + 1 beta tubulin
Beta-tubulin is a …?
GTPase
List a few microtubule associated proteins:
Kinesin
Dynein
Towards which end does Kinesin walk towards?
+
Towards which end does Dynein walk towards?
-
What do Dyneins need to walk?
ATP
What are Taxanes?
Microtubule-stabilizing agents
What is an example of a microtubule-destabilizing agent?
Vinca Alkaloids
True or False “Intermediate filaments need ATP or GTP”
False
Name a few intermediate filament associated proteins:
Plectin and nesprin
What is the size of septin diameter?
5nm
What are septins?
GTPase proteins
what is septin 9 used for?
Testing for colorectal cancer in blood test
What is septin 4 used for?
Testing for urothelial cancer using urine test
name two proteins associated with septin
BORG and cdc42
Actin pairs with…?
Septin
Microtubules pairs with ...?
Intermediate filaments
CDK1 is also known as…?
Cyclin B
CDK2 is also known as …?
Cyclin A and cyclin E
CDK4-6 is also known as …?
Cyclin D
Does WEE1 inhibit or activate CDK1?
Inhibit
What is WEE1?
A kinase
Fill in the blank. The cell cycle is regulated by the ……
Proteasome
What are APC/C Cdh1 AND APC/Cdc20
Ubiquitin Ligases
HPV protein E6 degrades
p53
HPV protein E7 binds to
Rb
At which check point is CDK1 (cyclin B present?)
G2/M checkpoint
Are G0 cells Ki67 positive or negative?
Negative
What is Senescence?
A permanent cell cycle arrest
Which filaments are polarized?
Actin and microtubules
What are CAF?
Cancer associated fibroblasts
What is the role of CAF?
facilitate the progression of metastatic tumors by depositing and remodeling the ECM
What is TEP?
Tumour-educated platelets
What is the role of TEP?
Platelets educated by the tumour to enable the circulation of cancer cells from the blood stream to the site of the tumour
What are the three hallmarks related to cancer cell death?
1.Sustaining proliferative signaling
Evading growth suppressors
Enabiling replicative immortality.
What is Myc?
Myc is a proto-oncogene