M3 HEMATOCRIT + M4 ESR

hematocrit

• the ratio of the volume of packed rbc to the whole blood and is manually determined by transferring blood to a plastic tube uniform bore. centrifuging, measuring the column of rbcs, and dividing by the total length of the column of rbcs plus plasma

50%

• normal ratio of hematocrit

40-55%

• hct value for men

36-48%

• hct value for women

45-60%

• hct value at birth

microhematocrit

• method in hematocrit determination

• it is reported either a percentage or in liters per liter

7-7.5 cm or 70-75 mm

• length of microhematocrit tube

10000-15000 g / 5 mins

• centrifugation in microhematocrit

trapped plasma

• this problem occurs only in manual procedure

• seen in macrocytic anemie, hypochromic anemia, spherocytosis, thalassemia, and sickle cell anemia

erythrocyte sedimentation rate

• ordered with other tests to detect and monitor the course of inflammatory conditions such as rheumatoid arthritis, infections, or certain malignancies

modified westergren esr

• most commonly used method

• recommended by clsi and international council for standardization in hematology

wintrobe esr

• when this method was introduced, the specimen used was oxalate-anticoagulated whole blood