UNFINISHED -[SEMINAR] - Urine and Body Fluid Analysis Automation

What are the e following tests can be performed in Semiautomated Urine Chemistry Analyzers

1. pH

2. Protein

3. Glucose

4.Ketone

5. Blood

6. Bilirubin

7. Urobilinogen

8. Specific Gravity

9. Color

10. Creatinine

11. Nitrite

12. Leukocyte

13. Protein to Creatinine Ratio

Semiautomated Urine Chemistry Analyzers requires the operator to?

1. Dip the reagent strip into a urine sample that has been mixed well.

2. Blot the strip to remove excess urine.

3. Place the strip onto the reagent strip platform.

4. Press the analyze/enter button

Designed for a high-volume urinalysis laboratory with user walk-away capability

Fully Automated Urine Chemistry Analyzers

Can load many labeled tubes of urine on a carousel or rack at one time with the capability to insert a stat sample during the run

Fully Automated Urine Chemistry Analyzers

Fully Automated Urine Chemistry Analyzers uses what principle?

Reflectance photometry

What are the e following tests can be performed in Fully Automated Urine Chemistry Analyzers?

SAME lang sa SEMI-AUTOMATED nadagdagan lang:

+ Clarity

+ Protein-low

Give example of analyzers that uses Digital Flow Morphology

Iris iQ200 microscopy analyzer

Give example of analyzers that uses Digital Microscopy

1. UriseD 2

2. UD 10

Give example of analyzers that uses FLOW Cytometry

1. Sysmex UF-5000

2. Sysmex UF-1000i

3. Arkray AUTION HYBRID

FLOW CYTOMETRY uses what dye?

polymethine dyes

It uses laser-based flow cytometry that measures forward light scatter, side scatter, fluorescence staining characteristics, and adaptive cluster analysis to identify stained urine sediment particles

SYSMEX UF-1000i

In SYSMEX UF-1000i, the stained urine sample passes through the flow cell, where it is hydrodynamically focused and presented to what laser?

RED semiconductor laser (635 nm)

How many nm is RED semiconductor laser?

635 nm

To perform SYSMEX UF-1000i automated microscopy, _______mL of __________________________ is aspirated into the instrument and divided into two channels: one for urine particle analysis and one for bacteria staining and detection (

4 mL of uncentrifuged urine

In the bacterial channel:

I. the diluent stabilizes the pH and lyses the nonbacterial particles, eliminating amorphous crystals.

II. The stain is specific to the DNA in a bacterial cell, eliminating any nonspecific staining of debris.

a. Statement I is correct

b. Statement II is correct

c. Both Statement are correct

d. Neither

a. Statement I is correct

Statement II - specific to the RNA dapat

FORWARD scatter measures the?

Size (length)

SIDE scatter measures the?

Granularity

Sysmex UF-1000i Fully Automated Urine Particle Analyzerresults are displayed as?

scattergrams

SYSMEX UF-1000 can store up to _____________patient results

10,000

What are the Flagged particles in Sysmex UF-1000i Fully Automated Urine Particle Analyzer

Pathological casts,

Crystals

Small round cells (renal tubular epithelial cells or transitional epithelial cells)

Sperm

Mucus

Yeastlike cells

an automated, modular, and scalable urinalysis system that combines urine flow cytometry with digital image analysis by integrating the UF-5000 Fully Automated Urine Particle Analyzer and the UD-10 Fully Automated Urine Particle Digital Imaging Device

Sysmex UN-2000 Automated Urinalysis System

UN eh United Nation = Combined hahah

uses a single-screen data

a. Sysmex UN-2000

b. SYSMEX UD-10

c. Sysmex UF-1000i

d. UF-5000

Sysmex UN-2000

a complementary digital imaging system designed to capture detailed images of urine particles in urine samples that require further review

SYSMEX UD-10

Sysmex UN-2000 is an automated, modular, and scalable urinalysis system that combines?

flow cytometry with digital image analysis

UF-5000 + UD-10

Yung dalawang mataas sa FLOW and DIGITAL (10 and 5000)

Sysmex UD-10 classifies urine particles in urine samples that require further review BASED on their?

SIZE

utilizes fluorescent flow cytometry coupled with a blue semiconductor laser to measure particle length, particle volume, particle internal complexity, and total amount of nucleic acid contained within the particle

UF-5000

For iQ 200 Automated Urine Microscopy Analyzer, how many digital photomicroscopic images are taken per sample.

500 digital photomicroscopic images

UF-5000 utilizes fluorescent flow cytometry coupled with a _________ __________ ___________to measure particle length, particle volume, particle internal complexity, and total amount of nucleic acid contained within the particle

BLUE semi-conductor laser (488 nm)

How many nm is BLUE semiconductor laser?

488 nm

In UF-5000, how many nm enhanced detection and differentiation of small particles?

488 nm

What Channel of UF-5000 stains elements with nuclear material, such as WBCs, epithelial cells, and bacteria?

Core channel (CR ch) - kasi nga may CORE yung mga to which is yung NUCLEUS

What Channel of UF-5000 stains and measures anucleate elements, such as RBCs, crystals, and casts?

Surface channel (SF ch) - surface ng cell yung i-i-stain nya

provides the information on the particles' ability to depolarize light. Through the detection of the depolarized light, it works to differentiate urine particles that are birefringent such as crystals.

Depolarized side scatter (DSS) detector:

uses digital flow morphology (imaging) and auto particle recognition (APR) to categorize and count urine particles automatically in uncentrifuged urine based on size, shape, texture, and contrast

Beckman Coulter's iQ200 Microscopy Analyzer

Beckman Coulter's iQ200 Microscopy Analyzer uses ______________________ and ______________________to categorize and count urine particles automatically in uncentrifuged urine based on size, shape, texture, and contrast

digital flow morphology (imaging)

auto particle recognition (APR)

Beckman Coulter's iQ200 Microscopy Analyzer is able to aspirate how many mL of urine?

1.3 mL

Beckman Coulter's iQ200 Microscopy Analyzer is able to take how many PICTURES?

500 pictures

iQ200 uses Auto Particle Recognition (APR) software=that pre-classifies urine particles in the photographs based on size, shape, texture, and contrast into 12 categories:

What are the 12 categories?

1. RBC

2. WBC

3. WBC clumps

4. Hyaline cast

5. Unclassified cast

6. Squamous epithelial cells

7. Non- squamous epithelial cells

8. Bacteria

9. Yeast

10. Crystals

11. Mucus

12. Sperm

TRUE OR FALSE

Beckman Coulter's iQ200 Microscopy Analyzer does not need CENTRIFUGATION of urine

True

77 Electronika UriSed 2 and 3 Pro Automated Urine Sediment Analyze perform automated microscopy with digital imaging using?

Auto image evaluation module (AIEM) software

AIEM meaning

Auto image evaluation module (AIEM)

What is the ADVANTAGE of UriSed 2?

zoom capability

requires a minimum of 2 mL of urine that is centrifuged in a special cuvette to produce a monolayer of urine sediment

- Advantage: zoom capability to view images, and interpretation of the images is similar to that of manual microscopic smears.

UriSed 2 (sedMax)

incorporates phase contrast microscopy in addition to brightfield microscopy to improve differentiation of elements, such as hyaline casts, RBC membranes, crystals, and yeast

UriSed 3 Pro

identify cells using flat-flow cell digital imaging technology and a trained neural network

FUS-100 and FUS-200 Analyzers

Flat = FUS

It uses Artificial imaging identification (AII) software to classify and quantify the cells and formed particles in the uncentrifuged urine

FUS-100 and FUS-200 Analyzers

FUS-100 and FUS-200 Analyzers uses _____________________________software to classify and quantify the cells and formed particles in the uncentrifuged urine

Artificial imaging identification (AII)

FUS-100 and FUS-200 Analyzers has a digital camera that is able to capture up to how many photos?

820 photos

samples are easily transferred from one instrument to the next, providing complete walkaway capability with minimal sample handling from sampling through results

can independently perform both physical and chemical testing, microscopy analysis, and a combination of both

AUTOMATED URINALYSIS SYSTEMS

Clinitek AUWi System and AUWi Pro System can load up to how many samples tubes?

200 sample tubes

Clinitek AUWi System and AUWi Pro System can complete up to how many tests per hour?

80 tests per hour

Clinitek AUWIi System is a combination of what 2 analyzers?

Clinitek Atlas System + Sysmex UF-1000i

What analyzer is consists of the iChemVELOCITY urine chemistry analyzer and the iQ200 urine microscopy analyzer?

iRICELL Urinalysis Systems

iRICELL Urinalysis Systems is consists of the____________________ and _______________

iChemVELOCITY.

iQ200

What is the MINIMUM volume of urine is required for iRICELL Urinalysis Systems?

4 mL

LabUMat 2 can be integrated with the?

UriSed 2 or UriSed 3 (77 Electronika)

What is the MINIMUM volume of urine is required for LabUMat 2 with UriSed 2 or UriSed 3 System?

3 mL of uncentrifuged urine

LabUMat 2 with UriSed 2 or UriSed 3 System can analyze up to how many TEST per hour?

240 tests per hour.

cassettes with urine testing strips and sediment cuvettes are loaded onto the instrument

2.8 mL of urine is required.

Cobas 6500 Urine Analyzer

What is the MINIMUM volume of urine is required for Cobas 6500 Urine Analyzer?

2.8 mL

BODY FLUID ANALYSIS AUTOMATION

ADVIA 2120i uses what principle?

1. Flow cytometry

2. Light scatter

3. absorbance

ADVIA 2120i approved for counting cells in what body fluids?

pleural fluids,

peritoneal fluids

peritoneal dialysates

Sysmex XN-Series Analyzers is cleared for analysis of?

CSF (XN-10, XN 20)

Synovial

Pleural

Peritoneal

Beckman Coulter's iQ200 Body Fluid Menu:

CSF

Synovial

Pleural

Pericardial

Peritoneal

Peritoneal Lavage

Peritoneal Dialysate

General Serous

What XN series is NOT capable of performing test for PERICARDIAL FLUID?

All (XN-10, XN-11, XN -20)

What XN series is NOT capable of performing test for CSF?

XN-11

What are the two dilutions of the body fluid specimen will be analyzed?

(1): Iris Diluent (

2): iQ Body fluids Lysing Reagent

Sysmex XN-Series Analyzers uses what technology for RBC-Body FLuid?

impedance counting principles enumerations

Sysmex XN-Series Analyzers uses what technology for WBC-Body FLuid?

flow cytometry

Sysmex XN-Series Analyzers Used _________________ as RBC lysing reagent

Lysercell WDF

Sysmex XN-Series Analyzers Used ______________to stain RNA and DNA in the nucleated cells

Fluorocell WDF