Unit 6 Antimicrobial chemotherapy

Antibiotic

a compound that could kill microorganisms whilst sparing the host

What is targeted by antibacterial agents

• Cell wall biosynthesis

• Membrane integrity

• Protein synthesis

• mRNA synthesis

• DNA synthesis/maintenance

• Intermediary metabolism

Antibiotics that target cell wall biosynthesis

• Cycloserine
• Fosfomycin
• Glycopeptides
• B-lactams

what is folate necessaary for

DNA synthesis

What inhibits folate

Nucleic acids

What else do nucleic acids inhibit

DNA gyrase and transcription of DNA/RNA

What subunits do bqacteria have

30s and 50s

How to inhibit 30S subunit

T.A for RNA binding and mistranslation

how to inhibit 50s subunit

C.M. Inhibit peptide bond formation

how to test for antimicrobial susceptibility

MIC - lowest concentration of antibiotics that inhibit visible growth

MBC - lowest concentration of antibiotic sufficient to provide a >3log in cell visibility