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11 Terms
1
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Test for starch
Add Iodine & it goes blue black
2
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Test for lipids
Emulsion test
3
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Test for Protein
Biuret test
4
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Test for reducing sugars
Benedicts test
5
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Test for a non reducing sugar
Basically just a longer Benedict's a standard Benedicts test must be performed first
6
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Process for emulsion test
Dissolve lipid in alcohol and add water, if positive it forms a milky white emulsion
7
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Process for Biurete
Dissolve sample in water and add Biuret solution, of protein present it turns from blue to purple
8
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Temperature for Benedict’s
Over 60c
9
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Process for Benedict’s test
Dissolve sample, add equal Benedict’s solution, heat for 5 mins, if reducing sugar present turns from blue to brick red
10
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Non reducing sugar process
After Benedict’s has come back negative dissolve new sample in water then add equal vol dilute HCl, heat for 5 min, neutralise with sodium bicarbonate till stops fizzing, add Benedict’s solution, heat form5 mins. Of positive turns from blue to brick red/orange