Food tests

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Last updated 4:59 PM on 11/6/22
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11 Terms

1
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Test for starch
Add Iodine & it goes blue black
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Test for lipids
Emulsion test
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Test for Protein
Biuret test
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Test for reducing sugars
Benedicts test
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Test for a non reducing sugar
Basically just a longer Benedict's a standard Benedicts test must be performed first
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Process for emulsion test
Dissolve lipid in alcohol and add water, if positive it forms a milky white emulsion
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Process for Biurete
Dissolve sample in water and add Biuret solution, of protein present it turns from blue to purple
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Temperature for Benedict’s
Over 60c
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Process for Benedict’s test
Dissolve sample, add equal Benedict’s solution, heat for 5 mins, if reducing sugar present turns from blue to brick red
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Non reducing sugar process
After Benedict’s has come back negative dissolve new sample in water then add equal vol dilute HCl, heat for 5 min, neutralise with sodium bicarbonate till stops fizzing, add Benedict’s solution, heat form5 mins. Of positive turns from blue to brick red/orange
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What is the HCl for
To hydrolyse bonds between dipepteides