Food tests
- Test for starch - Add Iodine & it goes blue black
- Test for lipids- Emulsion test * Process * Dissolve lipid in alcohol * Add water * If emulsion forms lipids present
- Test for Protein - Biuret test * Process * Dissolve sample in water * Add Biuret solution, if protein present it turns from blue to lilac
- Test for reducing sugars - Benedicts test * Dissolve sample * Add equal volume of benedicts solution * Heat at > 60C for 5 Mins * If reducing sugar present then it turns from blue to green/orange/brick red
- Test for a non reducing sugar - Basically just a longer Benedict's a standard Benedict’s test must be performed first and come back negative * Then after Benedict’s test with a new sample; * Dissolve sample in water * Add equal volume of dilute HCl * This hydrolyzes the bonds between dipeptides artificially * Heat at >60C for 5 mins * Neutralize it with Sodium bicarbonate until it stop fizzing * Add equal volume of benedicts solution * Heat at > 60C for 5 Mins * If positive then it turns from blue to green/orange/brick red
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