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Simple stain dye
Methylene blue
Charge on bacterial cells
Negative
Charge on dyes used
Positive
Purpose of aseptic technique
Prevents contamination
Target circle location on slide
Bottom of the slide
Tool for transferring bacteria
Inoculating loop
Flammable items in aseptic technique
Inoculating loop, open test tube
Step after air drying specimen
Heat fix
Purposes of heat fixing
Stick microbes to slide, kill bacteria
Target molecule in Gram stain
Peptidoglycan
Peptidoglycan in Gram positive bacteria
Thick, stains purple
Peptidoglycan in Gram negative bacteria
Thin, stains pink/red
Primary dye in Gram stain
Crystal violet
Decolorizer in Gram stain
95% alcohol
Counterstain in Gram stain
Safranin
Gram positive species
Staphylococcus aureus
Gram negative species
Escherichia coli
Error for Gram positive appearing negative
Over-decolorization
Target molecule in acid fast stain
Mycolic acid
Use of steam in acid fast staining
Yes
Acid fast positive color
Pink
Acid fast negative color
Blue
Primary dye in acid fast stain
Carbolfuchsin
Decolorizer in acid fast stain
Acid alcohol
Counterstain in acid fast stain
Methylene blue
Acid fast positive species
Mycobacterium smegmatis
Acid fast negative species
Streptococcus agalactiae
Error for acid fast positive appearing negative
Over-washing with acid alcohol
Name the shape.
Spirillum
Name the shape.
Coccus
Name the shape.
Bacillus
Name the stain.
Gram positive
Name the stain.
Gram negative
Name the arrangement and species.
Staphylococcus; Staphylococcus aureus
Name the arrangement and species.
Streptobacillus; Bacillus cereus
Name the arrangement and species.
Streptococcus; streptococcus agalctiae
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Flashcard (41)