mRNA Polyadenylation mRNA processing in eukaryotes
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Cleavage proteins
associate with Pol II during late stages of transcription
CPSF and CstF travel with the Pol II CTD
CPSF and CstF are transferred to the RNA
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mRNA is cleaved
at termination sequence, then 3’ end is polyadenylated
RNA is cleaved
poly A polymerase (PAP) adds As
(~200 As)
poly A binding protein binds—more polyA-binding proteins leads to a longer polyA tail (usually ~200As)
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Functions of polyadenylation
Protects against nucleases
Facilitates mRNA export from the nucleus
facilitates translation
Note 
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