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whats the mechanism of staining
histochemical stain (chemical rxn between potassium ferrocyanide + ferric ions)
ppb demonstrates?
iron in tissues (hemosiderin)
end colour results for ppb
ferric iron= prussian blue
background= pale red
ppb control
bone marrow
spleen
known pos ctrl
whats the best fixative to use when staining for iron for ppb and why
10% nbf bc it preserves ferric iron w/o leaching it from tissues
what fixatives should be avoided if gna stain ppb
avoid metal and acid
what is perls working sltn made of
potassium ferrocyanide + hydrochloric acid