Molecular Evolution

Define evolution

Descent with modification from a common ancestor, leading to genetic and phenotypic changes over time.

Natural selection

Process where variants that increase survival or reproduction become more common.

Fitness

The ability of an organism to survive and reproduce in its environment (often measured by reproductive success).

What is molecular evolution?

The study of how DNA, RNA, and protein sequences change over time, the forces shaping these changes (e.g., mutation, drift, migration), and how this impacts phenotype, function, and diversity.

What are the main mechanisms of molecular evolution?

Mutation

Selection

Genetic Drift

Gene Flow

Recombination

Gene Duplication

Mutation

Creates variation (errors, radiation, damage).

Selection

Non-random process favoring beneficial alleles.

Genetic drift

Random fluctuations in allele frequencies (stronger in small populations).

Gene flow (migration)

Movement of alleles between populations (can be adaptive, neutral, or maladaptive).

Recombination

Creates new allele combinations; may lead to duplication/deletion.

Reshuffles alleles during meiosis → creates new allele combinations.

Can bring together beneficial alleles or break apart harmful ones.

Mechanism for duplication and deletion (via unequal crossing over).

Gene duplication

Provides redundancy or new functions, leading to innovation.

Unequal crossing over during meiosis.

Retrotransposition (RNA copy reinserted into genome).

Whole-genome duplication (WGD) → entire set of chromosomes duplicated.

How do mutations drive evolution?

Introduce new alleles.

Lead to phenotypic variation (appearance, physiology, behavior).

Interact with the environment (selection acts on them).

Advantageous mutations may increase in frequency over generations.

What are the types of selection?

Positive selection

Negative/Purifying selection

Balancing selection

Sexual selection

Positive selection

Increases frequency of beneficial alleles (e.g., lactase persistence, CCR5Δ32 for HIV resistance).

Negative/Purifying selection

Removes harmful alleles (maintains conservation).

Balancing selection

Maintains diversity (e.g., heterozygote advantage in sickle cell).

Sexual selection

Traits improving mate attraction (e.g., peacock tails).

What is genetic drift and when is it most powerful?

Random changes in allele frequencies due to chance.

Strongest in small populations.

Can lead to fixation (allele becomes universal) or loss (allele disappears).

Examples: Bottlenecks, Founder effect.

What is gene duplication and what happens after it?

Duplication of a DNA sequence provides evolutionary opportunities:

• Nonfunctionalisation: One copy becomes a pseudogene.

• Neofunctionalisation: One copy gains a new function.

• Subfunctionalisation: Functions split between gene copies.

Mechanisms:

1. Unequal crossing over

2. Retrotransposition

3. Whole-genome duplication.

What is sequence conservation?

Regions highly preserved across species due to purifying selection.

• Highly conserved: Coding exons, splice sites.

• Intermediate: UTRs, promoters, terminators.

• Low conservation: Introns, 3rd codon positions, intergenic regions.

Conservation = functional importance.

How is sequence conservation useful?

Identifying functional DNA/protein regions.

Predicting protein structure and function.

Locating regulatory sequences.

Understanding disease mechanisms.

Inferring evolutionary relationships.

Sequence Divergence

Divergence is the process by which two or more populations, genes, or species accumulate differences in their DNA or protein sequences over time.

Causes: Mutations, natural selection, genetic drift, and reduced gene flow.

Measurement: Sequence divergence can be quantified by comparing nucleotide or amino acid differences.

Uses:

Helps estimate evolutionary time using molecular clocks.

Allows reconstruction of phylogenetic trees.

Reveals functional constraints (conserved vs divergent regions).

Explains how gene duplication leads to new functions (e.g., divergence of globin genes into fetal vs adult hemoglobin).

What is a phylogenetic tree and what is it used for?

A diagram showing evolutionary relationships between organisms, genes, or species.

Distance on the tree = degree of relatedness.

Built using mutation rates, selection pressure, and sequence comparisons.

Uses:

Reconstructing gene/species evolution.

Tracing disease origins (e.g., SARS-CoV-2 zoonotic origins).

What is the globin gene family and why is it important in molecular evolution?

Originated from: ancestral duplication which split into myoglobin + hemoglobin → α-like and β-like clusters.

Stage-specific expression: Embryonic → foetal → adult globins.

Paralogs retained: By subfunctionalisation and natural selection.

Includes pseudogenes as evolutionary relics.

Shows interplay of duplication, divergence, and selection.

How do haemoglobins change during development?

Embryo: HBZ, HBE1 → high O₂ affinity.

Foetus: HBA1/2, HBG1/2 → pull O₂ from maternal blood (normally silenced after birth).

Adult: HBB, HBD → optimized for tissue O₂ delivery (mutations here clinically important).

What causes Sickle Cell Disease (SCD)?

SNV in HBB gene: Glu6Val mutation.

Homozygous (HbSS) → SCD.

Heterozygous (HbAS) → carrier, usually healthy, resistant to severe malaria.

Mutation ~7300 years ago.

Common in malaria-endemic regions (Africa, Middle East, Mediterranean, India).

What is the link between sickle cell and heterozygote advantage?

HbAA: Healthy, but malaria vulnerable.

HbSS: Sickle cell disease, reduced survival.

HbAS: Carrier, healthy + malaria-resistant.

This balancing selection maintains the HbS allele in populations → classic case of evolution shaping human health.

Heterozygote advantage: Where individuals carry 2 different alleles for a gene have a higher fitness(survival) than individuals who are homozygous for either allele

What is a pseudogene?

A DNA sequence resembling a gene but nonfunctional due to mutations or lack of regulatory elements. Often arises from gene duplication and nonfunctionalisation.

What are homologs, orthologs, and paralogs?

Homologs: Genes related by descent from a common ancestor.

Orthologs: Same gene in different species, diverged by speciation (e.g., human HBB and mouse Hbb).

Paralogs: Duplicated genes in the same genome (e.g., human HBA and HBB).

Give examples of positive selection in humans.

Lactase persistence (LP): continued ability to digest lactose in adulthood.

CCR5-Δ32 mutation: confers resistance to HIV infection.

Antibiotic resistance in bacteria.