peptides and proteins

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4 Terms

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Biuret test

  • is positive for a peptide or protein with two or more peptide bonds

  • Blue color of a basic solution of Cu2+ turns to a violet color when a tripeptide or larger peptide is present

  • Individual amino acids and deceptive do not react with reagent and will remain blue (negative)

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ninhydrin test

  • used to detect amino acids and most proteins

  • In the test most amino acids produce a blue violet color

  • Proline and hydroxyproline give a yellow color

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Xanthoproteic test

  • test is specific for amino acids that contain and aromatic ring

  • Yellow colored products

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