Infection Sciences Flashcards

Compound light microscope

A ________ is an optical microscope that uses visible light and different lenses to magnify an object.

Intubation

________: insertion of tube into body canal or hollow organ e.g. stomach specimens

external structures

Fixing It is the process by which internal and ________ are preserved and fixed in position.

Catheter

________: tubular instrument used to withdraw or introduce fluids into body cavity e.g. urine

smear

Drying The ________ is air- dried before fixing.

overall morphology

It preserves the ________ but not the internal structures Chemical fixing.

fine cellular substructures

It protects ________ and morphology of larger, more delicate organisms.

Clean catch

________ method: collection of midstream portion of urine 15 Transport of specimen  Should be timely  May involve use of special media that preserve microbes in specimen

* Temperature control may be required
* Swabs and transport media are used
* Some swabs may need supplementation to support microbial survival or inhibit microbiota growth
* Antibacterial compounds (e.g., penicillin) can be used to ensure fungi recovery
* Goals of preservation: sustain microbial viability, prevent contaminant growth
* Thioglycolate and cysteine are typically used in anaerobic transport media to reduce oxygen levels.

needle

When the ________ is in place, fluid can drip out into a collection vial.

Highest magnification

________ is x1000 (x10 x100) 20 Magnification power To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.

Intubation

insertion of tube into body canal or hollow organ e.g

Catheter

tubular instrument used to withdraw or introduce fluids into body cavity e.g urine

Clean-catch method

collection of midstream portion of urine 15 Transport of specimen  Should be timely  May involve use of special media that preserve microbes in specimen -

* Temperature control may be required
* Swabs and transport media are used
* Some swabs may need supplementation to support microbial survival or inhibit microbiota growth
* Antibacterial compounds (e.g., penicillin) can be used to ensure fungi recovery
* Goals of preservation: sustain microbial viability, prevent contaminant growth
* Thioglycolate and cysteine are typically used in anaerobic transport media to reduce oxygen levels.

Microscopy Choice of smear (how the specimen is prepared on the microscope slides)

**Wet-mount** (unfixed and unstained Eg. Urine &CSF) **Heat-fixed** • **Chemically fixed** (Alcohol/acetic acid solution)

Bright - field microscope

* The name "bright-field" comes from the bright viewing field that contrasts with the dark specimen being observed.
* It uses visible light for illumination.
* The microscope produces an image of the object being observed against a bright background.

Types of fixing

**Heat fixing** - It preserves the overall morphology but not the internal structures **Chemical fixing** - It protects fine cellular substructures and morphology of larger, more delicate organisms. E.g. alcohol/acetic acid solution