Histo Lab- Lymphatic System (Aughey)

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Lymph node (dog). (1) Connective tissue capsule. Cortex with (2) lymphatic follicles and (3) paracortex. Masson’s trichrome. ×20.

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Lymph node (sheep). (1) Connective tissue capsule. (2) Connective tissue trabeculae. (3) Lymphatic tissue. H & E. ×62.5.

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Lymph node (cat). The reticular fibres form a black network and support the cells of the lymphatic tissue. Gordon and Sweet. ×100.

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Lymph node (dog). (1) Connective tissue capsule with some fat cells. (2) Subcapsular sinus. (3) Cortical follicle. (4) Parafollicular tissue. (5) Sinusoid. H & E. ×125.

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Lymph node. Cortex (dog). A single follicle is present. The central zone is the pale staining reactive germinal centre. There is an outer rim of closely packed small lymphocytes. H & E. ×125.

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Lymph node. Cortex (dog). The germinal centre is composed of lymphoblasts, reticular cells and macrophages. H & E. ×125.

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Lymph node. Paracortex (pig). (1) Connective tissue capsule. (2) Connective tissue trabeculae. (3) Subcapsular sinus. (4) Thymus-derived T lymphocytes. (5) Sinusoid. Alcian blue. ×125.

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Lymph node. Medulla (dog). (1) Loose aggregation of lymphoid tissue. (2) Open meshwork of sinusoids. H & E. ×250.

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Lymph node. Medulla (dog). (1) Sinusoid lined by macrophages. Also, clusters of small lymphocytes (arrowed). Alcian blue. ×125.

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Lymph node (cat). Anti-CD4 monoclonal antibody. The avidin/biotin method detects an exclusive population of T lymphocytes in the parafollicular and deep cortex of the lymph node; brown reaction. Avidin/biotin method. ×62.5.

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Lymph node (cat). Anti-CD4 monoclonal antibody. The avidin/biotin method detects an exclusive population of T lymphocytes in the parafollicular and deep cortex of the lymph node; brown reaction. Avidin/biotin method. ×125.

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Lymph node. Cortex. Anti CD8/CD4 monoclonal antibody. This subset of positive cells are rarely present in the follicle germinal centre. Avidin/biotin method. ×100.

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Lymph node. Cortex. Anti-C.D.21 monoclonal antibody. This method recognizes the follicular dendritic cells and the cell processes; stained red. Avidin/biotin method.×62.5.

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Haemal lymph node (ox). (1) Connective tissue capsule. (2) Blood filled sinusoids. H & E. ×20.

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Spleen (horse). (1) Fibromuscular capsule. (2) Fibromuscular trabeculae. (3) Splenic pulp. H & E. ×12.5.

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Spleen (horse). (1) White pulp. (2) Red pulp. (3) Trabecula. H & E. ×62.5.

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Spleen (dog). (1) White splenic corpuscle with an eccentric arteriole (arrowed). (2) Sinusoids of the red pulp filled with erythrocytes. H & E. ×125.

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Spleen (cat). (1) Part of a trabecula. (2) Ellipsoid. (3) Blood filled sinusoids of the red pulp. H & E. ×250.

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Spleen (dog). The site of iron deposits as a result of erythrocyte phagocytosis are blue. Perls’ Prussian blue. ×125.

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Spleen (dog). The marginal zone has a large population of macrophages. These are shown filled with phagocytosed carbon particles. Toluidine blue. ×250.

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Thymus (ox). (1) The supporting epithelial cells derived from the pharyngeal endoderm. (2) Thymocytes. (3) Thymic corpuscle. H& E. ×125.

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Thymus (ox). (1) Fine connective tissue capsule. (2) Fat laden interlobular connective tissue. Thymic lobule with (3) outer cortex of densely packed thymocytes, and (4) inner less cellular medulla. H & E. ×62.5.

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Thymus (ox). (1) Thymocytes in the cortex. (2) Medulla with epithelial cells and few thymocytes. H& E. ×125.

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Thymus (ox). The small dark blue cells are the densely packed cortical thymocytes. H & E. ×250.

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Thymus (ox). The central medullary zone is less cellular; a thymic (Hassall’s) corpuscle is arrowed. H & E. ×125.

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Fetal thymus (ox). (1) The connective tissue of the capsule and the supporting trabeculae; there are no fat cells. Thymic lobules with (2) cortex and (3) medulla. H & E. ×62.5.

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Fetal thymus (ox). (1) Connective tissue capsule. (2) Cortical zone of the thymic lobule with loosely arranged thymocytes. The intercellular supporting framework is composed of epithelial cells. H & E. ×125.

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Tonsil (dog). (1) Stratified squamous epithelium. (2) Dense aggregation of lymphoid tissue in the lamina propria. H & E. ×20.

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Tonsil (dog). (1) Stratified squamous epithelium. (2) Connective tissue lamina propria. (3) Lymphoid tissue. H & E. ×125.

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Tonsil (dog). (1) Stratified squamous epithelium lining the crypt. (2) Dense aggregations of lymphoid tissue in the lamina propria. (3) Mucus-secreting salivary glands. H & E. ×7.5.

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Stomach (cat). A lymph nodule is present in the mucosal/submucosal layer of the stomach. H & E. ×7.5.

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Abomasum (goat). A lymph nodule in the lamina propria of the mucosa. H & E. ×62.5.

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Cloacal bursa (bird). (1) Simple columnar epithelium of the cloaca. (2) Cortical area of densely packed lymphocytes. (3) Sparsely populated medulla. H & E. ×62.5.

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Cloacal bursa (bird). (1) Simple columnar epithelium of the cloaca. (2) Connective tissue lamina propria. (3) Cortex. (4) Medulla. H & E. ×125.

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Bone marrow specimen from an iguana. This section contains several macrophages with engulfed cellular material and bacteria. Note the green inclusion within the large macrophage which contains biliverdin, the degradation product of haemoglobin in amphibians, reptiles and birds. H & E. ×250.

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Section of cortical part of a thymic lobule from a desert tortoise (Xerobates agassizi). (1) Epithelial cells. (2) Concentric (Hassall’s) corpuscle. (3) Thymocytes. H & E.×125.

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Loose connective tissue (dog). The histiocytes have phagocytosed the injected carbon particles (arrowed). ×250.

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Liver (sheep). The macrophages lining the sinusoids have phagocytosed the injected carbon particles (arrowed). Safranin/haematoxylin. ×250.