The compound microscope ; magnification and resolution

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21 Terms

1
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the compound microscope is

An instrument for magnification of the angle of sight of small objects invisible to the unaided eye

2
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The mechanical system consists of

base and arm, object stage, tube, rotating turret for objective lenses, fine and coarse focus adjustment knobs

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An illumination system consists of

a light source, collimator, condenser, and diaphragms for shaping the illuminating light

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The image created by the objective lens is

Enlarged, inversed, real

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The image created by the eyepiece is

enlarged, upright, virtual

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Transverse magnification of the component of the optical system

the ratio between a transverse dimension of the image and the corresponding transverse dimension of the object

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Microscope magnification is

the product of the transverse magnification of the objective lens, the transverse magnification of the eyepiece

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Two images are considered separate if

the distance between their centers is not less than their radius

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The resolution limit of the compound microscope is

the smallest distance between two points of the object at which they are imaged separately

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The aperture angle of the objective front lens is the

The half - angle of the maximum cone of light originating from the object and entering the objective lens

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The resolution limit can be reduced by using

objectives with a larger numerical aperture and light with shorter wavelength

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The effective aperture angle of the immersion objective lenses becomes

larger and the resolving power increases

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The image for the bright field microscopy is produced by

light transmitted or reflected by the object

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The image for the dark field microscopy is produced by

light scattered by the object

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Phase contrast microscopy is used for

imaging of low contrast objects

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An illumination system provides the required …….. ,…….. , ……….. of the illuminating light beam

intensity, diameter, convergence

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The front lens of the objective determines the …….. and the remaining lenses correct the

magnification, aberrations

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The eyepiece concsists of two or more lenses . The …… lens determines the ….. of the eyepiece

upper , magnification

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Objective magnification depend on the ….. and the …..

focal length of the objective lens; optical length of the tube

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Eyepiece magnification depends on the ………. and the ……

focal length of the eyepiece; normal near point distance for the eye

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as a result of diffraction of light , the image of a point is a ………. surrounded by …….

disk ; diffraction rings