Intro to Histology

What are cells?

smallest functional and structural units of all organisms

What is an example of a cell?

nucleus, cytoplasm, organelles, enclosed in a membrane

What are tissues?

consist of morphological and functionally similar cells and various amounts of intercellular material

What are examples of tissues?

epithelial, connective, muscle, and nervous

What is bright field microscopy?

stained live cells and tissues are observed due to differences in color and intensity

What is the most common application in bright field microscopy?

used for everyday histopathology

What are the different types of electron microscopy?

transmission and scanning

What is transmission EM?

interactions with electrons to form a 2D image

What is scanning EM?

interactions with electrons to form a 3D image

What is the most common application in electron microscopy?

virology for virus detection

What is fluorescent microscopy?

cells and tissues stained or labeled with fluorescent antibodies

What is the most common application in fluorescent microscopy?

virus detection

What is virtual microscopy?

scanned digital image

What is the most common application in virtual microscopy?

used for teaching

Who invented the microscope?

Zacharias Janssen

Who has the credit for discovering the cell?

Robert Hooke

What does HE stand for in the stain?

heamtocylin and eosin

What is HE stain used for?

everyday histopathology

What is heamtoxylin and what color does it stain?

basic dye that binds to acid to stain blue

What is eosin and what color does it stain?

protein rich acid that binds to base and stains red

What is masson trichrome used for?

detection of fibrocollagenous tissue

What stains what color in masson trichrome staining?

proteins stain red and collagen stains blue

What is the periodic acid schiff stain used for?

yeast detection