Chap 4: The Microscope

All Parts of a Microscope

All Parts of a Microscope

Eyepiece lens, Nosepiece, objective lens, clips, stage, condenser, mirror/light source, fine adjustment, coarse adjustment

The Eyepiece lens

Magnifies the image

Nosepiece

Revolves to move the desired lens into position

Objective lens

Magnifies the image. There are three objective lenses, scanning lens with 4x, low power with 10x, and high power with 40x

Clips

Hold the slide in place

Stage

Holds the slide which contains the specimen

Condenser

Focuses light on the object

Mirror or light source

Supplies light to the object

Fine adjustment

Typically smaller knob used for precise focusing

Coarse adjustment

Typically larger knob used for rough focusing

Total magnification

The magnifying power of the eyepiece lens x the magnifying power of the objective lens. E.g. Eyepiece= 10x Objective= 4x Total magnification= 40x

Using a microscope

Start of lowest magnification, place slide on stage and lock with clips, adjust light source, use coarse adjustment, use fine adjustment, view

Types of Microscope

Light Microscope, and electron microscope

Light Microscope

Sends a beam of light through the cells, which is then focused by the two lenses, the eyepiece and the objective.

Electron Microscope

Sends a beam of electrons through the cells, focuses them with electromagnets to illuminate the specimen