12 stains and microscopy

0.0(0)
studied byStudied by 0 people
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/11

encourage image

There's no tags or description

Looks like no tags are added yet.

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

12 Terms

1
New cards

Sternheimer malbin

  • general use stain

  • crystal violet and safranin O

  • delineates nucleus and cytoplasm.

    Used for WBC, epithelial cells, and casts (purple).

2
New cards

Toluidine blue

  • used to enhance nuclear detail

  • used to distinguish WBC from RTE

<ul><li><p>used to enhance nuclear detail</p></li><li><p>used to distinguish WBC from RTE</p></li></ul><p></p>
3
New cards

Lipid stain

  • Oil red O or Sudan III

    • red

  • Stains triglycerides and neutral fats

    • NOT CHOLESTEROL

4
New cards

Gram stain

mainly used in UA for identifying bacterial casts

5
New cards

Hansel stain

  • identify eosinophils

  • methylene blue and eosin Y

  • in acute interstital nephritis

6
New cards

Prussian blue

  • used to see hemosidorin (iron) granules in casts or RTE cells

7
New cards

2% acetic acid

  • dissolve amorphous phosphates

  • enhance nuclear detail of WBC

  • lyse RBC, not bacteria or yeast

8
New cards

2% sodium hydroxide

  • dissolve amorphous urates, better visibility of bacteria

  • destroy cells and casts

9
New cards

Bright field

used for routine UA

10
New cards

Phase contrast

enhances low refractive objects

11
New cards

Polarizing

will cause birefringence in certain elements

  • cholesterol and certain crystals

12
New cards

Interference contrast

three dimensional look by increasing detail/contrast of image