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Kreb’s cycle
Step 2 of Cellular respiration
Oxidization of pyruvate - prep for Kreb’s cycle
Location: mitochondrial matrix
Enzymes: kinase, dehydrogenase
Produces: 2ATP, 8NADH, 2FADH2, 6CO2
Aerobic
With oxygen
Anaerobic
Without oxygen
Oxidative phosphorylation
Last stage of cellular respiration
Connects rxns of ETC and the flow of H+ through ATPsynthase (chemiosmosis)
Alcohol fermentation
Converts pyruvate into ethanol and CO2
Acetyl CoA
Other product when oxidizing pyruvate, enters the Kreb’s cycle to convert into energy
2 Carbon acetyl group
Cellular respiration
Converting glucose into usable energy
glucose + oxygen → water + CO2 + ATP
Redox reactions
passing of e-
cellular respiration
glucose → NADH/FADH2 → integral proteins → O2 → H2O
photosynthesis
H2O → chlorophyll → PEA → NADPH → G3P → glucose
ATP synthase
enzyme that produces ATP out of ADP
setup H+ gradient
allows p+ to flow through ATP synthase
Substrate-level phosphorylation
making ATP
kinase is the enzyme that catalyzes the transfer of a phosphate group to ADP to create ATP
Lactic acid fermentation
pyruvate (3C) → lactic acid (3C)
Oxidation
loss of e-
Glycolysis
Step 1 of Cellular Respiration
Breaking down of glucose into 2 pyruvates
Location: cytoplasm
Enzymes: kinase, dehydrogenase
Produces: 4ATP and 2NADH
Consumes: 2ATP
First 5 rxns - invests ATP
Last 5 rxns - harvests a little ATP and a little NAD
*NET YIELD = 2ATP and 2NADH
Catabolism
the breaking down of complex molecules into simpler ones, releasing energy in the process
glycolysis is an example of a catabolic rxn
Reduction
gain of e-
Chemiosmosis
diffusion of ions across a membrane
build up of p+ gradient so that H+ can flow through ATP synthase enzyme to build ATP
NAD+/NADH
e- carrier
proton donor
Proton motive force
gradient between inter-membrane space and the matrix
Proton gradient
difference in p+ concentration across a membrane
Photosynthesis
life from light and air
CO2 + water + light → oxygen + glucose
Non-cyclic photophosphorylation
6 steps
making ATP
H2O splits to give Chl A new e- (makes O2)
high energy e- stored in NADPH
Autotrophs
plants
make own energy
energy from sunlight
build organic molecules from CO2
Cyclic electron flow
e- returns to OG Chl A
stays in the same photosystem
Heterotrophs
animals
energy from eating others
respiration
Cyclic photophosphorylation
produces ATP without producing oxygen or NADP
Chlorophyll
absorbs light in photosynthesis
causes e- jump in energy
Absorption spectrum
plot that shows the wavelengths of light that a substance absorbs
Chlorophyll A absorbs best in red and blue, least of green
Photosynthesis gets energy by absorbing wavelengths of light
Mesophyll cells
where most of photosynthesis takes place
Chlorophyll a
absorbs best in red and blue, least of green
absorbs wavelengths to produce energy in photosynthesis
light absorbers in photophosphorylation
Rubisco
enzyme that fixes carbon from air
makes life out of air
most abundant enzyme
Stomata
facilitates gas exchange, allows CO2 to enter and O2 to exit
Action spectrum
relative rate of photosynthesis at different wavelengths of light
C3 plants
first stable product of photosynthesis is 3 carbon compound
Stroma
location of Calvin cycle
after light rxns
convert CO2 into glucose
Chlorophyll b
accessory pigment
expands range of light/wavelengths a plant can capture
Photorespiration
oxidization of RuBP → pulls e- out (breaking sugars)
no ATP gain
Light reactions
light dependent
energy conversion from solar → chemical (ATP and NADPH)
Accessory pigments
different structures absorb light of different wavelengths
C4 plants
better way to capture CO2
carbon fixation with PEP carboxylase, stores as 4C compound
adaptation to hot, dry climates
Calvin cycle
adds energy
location: stroma (chloroplast)
transforms CO2 into glucose
light rxn products to drive synthesis rxns
Carbon fixation
Reduction
Regeneration of RuBP
end product: energy rich 3C sugar
Bundle-sheath cells
a layer forms a sheath around the veins in leaves
transports water and nutrients
helps with carbon fixation in C4 plants
NADP+/NADPH
e- carrier
proton donor
found in photosynthesis
Reaction center chlorophyll
core of photosynthesis, chlorophyll molecules that capture light and initiate photosynthesis
Photophosphorylation
making ATP from light
Carbon fixation
process to convert CO2 into organic molecules like glucose
first step in calvin cycle
Rubisco fixes carbon
PEP carboxylase
close in the day - releases O2 from 4C acids to Calving cycle, increases concentration of CO2 in cells (open
open at night - open tomatoes and fix carbon in 4C “storage” compounds (fill)
Photosystem I
uses light energy to create energy carrying molecules
Photosystem II
protein complex that captures light energy to split up H2O
CAM plants
adaptation to hot and dry climates
separate carbon fixation by time
contains PEP carboxylase
Thylakoid membrane
inner membrane of the chloroplast
location of phosphorylation in photosynthesis
location of etc in photosynthesis