ATP Synthesis

What uncouples ETC from ATP synthesis?

2,4-DNP, thermogenin (UCP1 in brown fat), aspirin in overdose

How many ATP per NADH and FADH₂?

NADH → 2.5 ATP,

FADH₂ → 1.5 ATP

What part contains the catalytic subunits?

F1

How many protons per ATP formed?

3 H+

What does the aspartic acid residue serve as in complex V?

Binding Site and Movement can only happen if Asp is bound

What generates the synthesize of ATP?

ETC generates a proton gradient

Define this enzyme:

ADP enters only if ATP exits

14% of proteins in the inner mitochondrial membrane

ATP is released more quickly in matrix

ATP-ADP translocase

Define this enzyme:

exchange ATP for ADP

Reduces the H+ gradients (cost one more proton)

Adenine nucleotide metabolism