ferritin and beer lambert law

what is ferritin?

iron-storage proteins

what is apoferritin?

when the protein shell is empty and contains no iron

how many protein subunits does ferritin have?

24

how much can apoferritin hold?

4500 iron atoms

what are four-fold channels?

type of channel that occurs at the intersection of four protein subunit

what are three-fold channels?

type of channel that occurs at the intersection of three protein subunits

what does the measurement of absorbance tell us about a solution?

can be used to obtain the concentration of the species in the solution

what is incident light?

Io, and is the amount of monochromatic light that is passed through the sample

what is transmitted light?

the amount of light monitored by the detector

what is transmittance?

the ratio of the intensity of transmitted light (I) to that of the incident light (Io)

the relationship of absorbance and b and c of the beer-lambert law

proportional to the concentration of the absorbing species © and the path length of the light (b)

what is the proportionality constant?

extinction coefficient of the solute at a given wavelength

when can the bee lambert law not be used

for solutions of high concentrations

for most accurate results must be 0.15 and 0.85

what does it mean when a solution is more intense in color?

it absorbs a greater quantity of light in the visible region — which means it has a greater extinction coefficient

with what can ferrozine only form a complex with?

Fe²+ not Fe³+