Wright Stain/Heinz Bodies
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11 Terms
Components of stain dissolved in methanol
Methylene blue (positive ions, stains DNA/RNA)
Azure B (works with M. blue)
Eosin Y (negative ions, stains cytoplasmic structures)
Buffer of Wright Stain causes…
Ionization of the dyes
Presence of precipitation indicates…
Inadequate filtration of stain; replace stain
Heinz Bodies…
Represent unstable hemoglobin that are denatured/precipitated
Supravital stains are used for…
Heinz Bodies
Heinz Bodies are not visible on…
Wright Stain
When glycolytic enzymes in RBCs are unable to prevent hgb oxidation they form…
Heinz Bodies
What are Heinz Bodies indicative of?
Injury to RBCs via oxidant drug, G6PD deficiency, unstable hemoglobins and alpha thalassemia
When are Heinz Bodies most commonly seen?
After oxidant challenge of post-splenectomy
What stain is used for supravital stains?
Crystal violet
How do Heinz Bodies look on supravital stains?
Blue, refractile inclusions often found close to RBC edge
Note 
Flashcards (319)