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Microbial Growth
increase in cell size or number of cells
Growth
Increase in the number of cells
Binary Fission
cell division following enlargement of a cell to twice its minimum size
Generation Time
Time required for microbial cells to double in number
Chromosome
During cell division, each daughter cell receives a ______ and sufficient copies of all other cell constituents to exist as an independent cell
Cell elongates and DNA is replicated
What’s the first step of binary fission?
Cell wall and plasma membrane begin to constrict
What’s the second step in binary fission?
Cross-wall forms, completely separating two DNA copies
What is the third step in binary fission?
Cells separate
What is the fourth step in binary fission?
Cell wall
What is the outer layer of the cell?
Plasma membrane
What does the inner layer of the cell consist of?
DNA
What does the cell consist of?
Binary fission
an asexual reproduction method where a single cell divides into two genetically identical daughter cells
Binary fission
What is this an example of?

Divisomes
Fts proteins interact for form ____
FtsZ
_____ molecules form a ring around the center of the cell 7 attracts other proteins
Divisome
a protein complex in bacterial cells responsible for cell division.
Generation time
The time it takes one generation to form the next generation
Double
Generation times ____ in number
Shorter
Mist bacteria have _____ generation times than eukaryotic microbes
Growth
Generation time is dependent on ____ medium and incubation conditions
Incubation
Generation time is dependent on growth medium and _____ conditions
Nt=N0×2^n
The equation for generation times
The red line
Which like best represents the generation time?

Batch-culture
A closed-system microbial culture of fixed volume
Continuous culture
An open-system microbial culture of fixed volume
Chemostat
The most common type of continuous culture device
Lag phase
Interval between when a culture is inoculated and when growth begins
Exponential phase
Cells in this phase are typically in the healthiest state
Stationary phase
The growth rate of the population in this phase is at zero
Stationary phase
In this phase, either an essential nutrient is used up or water product of the organism accumulates in the medium
Death phase
In this phase, if incubation continues after cells reach stationary phase, the cells will eventually die
70%
The CDC estimates that about ___ Of human bacterial infections involve biofilms.
Biofilms
Most nonsocomial infections on medical catheters are caused by ______
84-421 days
How many days after exposure do pateients develop biofilms infection?
Culture Media
Nutrients prepared for microbial growth
Sterile
No living microbes
Inoculum
Introduction of microbes into medium
Culture
Microbes growing in/on culture medium
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