Biochemistry - Tissue Biochemistry

Which one of the following is the most potent ROS:

Hydroxyl radical

Which enzyme generates hydrogen peroxide?

Superoxide dismutase

Which amino acid of glutathione is the free sulfhyrdryl group (-SH) located on?

Cysteine

Which 3 amino acids make up glutathione?

Glutamate, Cysteine and Glycine

What are the 3 antioxidant enzymes that protect against ROS?

Superoxide dismutase, catalase and glutathione peroxidase

Intracellular levels of GSH in mammalian cells:

0.5 - 5mM

Extracellular concentrations of GSH:

2-20 um

Where is GSH synthesised?

Intracellulary in the cytoplasm

Which enzyme catalyses the rx between glutamate and cysteine to form y-glutamyl-cysteine?

Y-glutamylcysteine synthetase

What enzyme catalyses the rx when glycine is added to y-glutamyl-cysteine?

Glutathione synthetase