Lecture 14 pt.2 - Kinetics

What is K_off and K_on

K_off - off rate or dissociation rate

K_on - on rate or association rate

What is the rate of forward and reverse reaction

forward = [A][B]k_on

Reverse = [AB]k_off

Michaelis-Menten

rapid equilibrium assumption

rapid equilibrium assumption: breakdown of enzyme-substrate complex to form products is much slower than the velocity to form the complex

Michaelis-Menten

is the formation of products reversible?

what is the assumption about the product?

formation of products is irreversible, and the assumption is there is little to no product

Michaelis-Menten

The formation of products ___ is the ___ _____ and thus

the formation of products (k2) is the slow step and thus is the rate limiting reaction

Michaelis-Menten

what is the plot?___ vs ____

when is velocity at Vmax?

what happens if the substrate is less

the plot is velocity vs substrate

velocity is a Vmax when the substrate is greater than the dissociation constant

fi the substrate is much less than the plot is linear

What is the exception to Michaelis-Menten?

What kind of kinetics do they have?

Allosteric enzymes are an exception because they have more than toe submits and active site. they do not have Michaelis-Menten kinetics but rather sigmoidal kinetics

Briggs and Haldane

what is the the steady state assumption

the steady state assumption is the velocity is more generalized because the enzyme substrate complex reaches a more constant value, meaning equilibrium

ES formation = ES breakdown

Briggs and Haldane

when does Km=Ks

Km=Ks when k-1 is much greater than k2

What is Vmax

What is Km

Vmax is the maximum velocity of the reaction when the substrate is striated in solution

Km refers to affinity and is the substrate concentration at half Vmax