Week 9-Follow up

What do you add to the Indole test?

Kovac’s reagent to detect the indole and see if it produces a pink to red later on the surface

What do you add into the methyl test?

methyl red solution to see if the organism produces mixed acids

what do you had to the Voges Proskauer test?

add Barrett’s A (a-naphthol reagent) and Barrett’s B ( potassium hydroxide) to indicate the presence of acetoin

How to know if the Citrate test is positive?

if there’s a development of bright blue color indicating positive for citrate permease production and citrate utilization.

what does gas production indicate in the Nitrate test?

the bacterium reduced the nitrate to gaseous products of nitrogen

what does no gas production indicate in the nitrate test?

reagent A (sulfanilic acid) and reagent B (dimethyl alpha-naphthylamine) are added and test for the reduction of nitrate to nitrite