Kreb's Cycle Steps | Quizlet

1. Acetyl CoA + Oxaloacetate

Citrate via citrate synthase

2. Citrate

Isocitrate via aconitase

3. Isocitrate

alpha-ketoglutarate + CO2 via isocitrate dehydrogenase

4. alpha-ketoglutarate

Succinyl CoA + CO2 via alpha-ketoglutarate dehydrogenase

5. Succinyl CoA

Succinate + CoA via succinyl CoA synthetase

6. succinate

fumarate via succinate dehydrogenase

7. fumarate

malate via fumarase

8. malate

oxaloacetate via malate dehydrogenase

In which steps does decarboxylation occur?

3, 4 (substrates: isocitrate and alpha-ketoglutarate)

In which steps are NADH + H+ made?

3,4,8 (substrates: isocitrate, alpha-ketoglutarate, and malate)

In which step is FADH2 made?

6 (substrate: succinate)

In which step is GTP made?

5 (substrate: succinyl CoA)

What is the intermediate of step 2 (citrate->isocitrate)?

cis-aconitate

What is the intermediate of step 3 (isocitrate->alpha-ketoglutarate)?

oxalosuccinate

What is the net reaction of the PDC?

Pyruvate + NAD+ + CoA ---> Acetyl CoA + NADH + H+ + CO2

what is the net reaction of the Kreb's cycle?

Acetyl CoA + 3 NAD+ + FAD + GDP + Pi + 2H2O ---> CoA + 2CO2 + 3NADH + 3H+ + FADH2 + GTP

Which steps of the Kreb's cycle use H2O?

1, 7