LABORATORY REVIEWER

Most preffered site for VENIPUNCTURE

Median cubital

2nd prefered site for VENIPUNTURE

Cephalic Vein

Vein located at the lateral side

Cephalic Vein

Vein located at the dorsal side

Basillic vein

Blood Culture tube
Coagulation tube
Serum tube
Heparine tube
Edta tube
Sodium flouride tube

Which of the following blood collection tubes contains sodium polyanethol sulfonate

Blood culture tube

Blood Culture tube
Coagulation tube
Serum tube
Heparine tube
Edta tube
Sodium flouride tube

Which of the following blood collection tubes contains Buffered sodium citrate

Coagulation tube

Blood Culture tube
Coagulation tube
Serum tube
Heparine tube
Edta tube
Sodium flouride tube

Which of the following blood collection tubes is clot activator with gel

Serum tube

Blood Culture tube
Coagulation tube
Serum tube
Heparine tube
Edta tube
Sodium flouride tube

Which of the following blood collection tubes contains Acid citrate dextrose

Blood Culture tube

Blood Culture tube
Coagulation tube
Serum tube
Heparine tube
Edta tube
Sodium flouride tube

Which of the following blood collection tubes contains CTAD

Coagulation tube

Blood Culture tube
Coagulation tube
Serum tube
Heparine tube
Edta tube
Sodium flouride tube

Which of the following blood collection tubes contains Lithium heparin

Heparin tube

Order of Draw 1/6

Blood culture tube (SPS , ACD)

Order of Draw 3/6

Serum Tube

Order of Draw 2/6

Coagulation tube ( Buffered Sodium Citrate , CTAD )

Order of Draw 5/6

EDTA tube

Order of Draw 4/6

Heparin tube

Order of Draw 6/6

Sodium flouride tube

Inversion of CTAD

3-4x

Inversion of Thrombin-based Clot activator

5-8x

Inversion of Silicon coated with clot activator

5x

Used as primary stain in gram staining

Crystal violet

Used as Mordant in Grams staining

Grams iodine

Used as Counter stain in Gram’s staining

Safranin red

Prefered specimen for Sputum

First morning

Mordant for Zeihl-nelsen Acid fast staining

Heat

Mordant for kinyoun method Acid fast staining

Tergitol

Counter stain used for acid fast staining

Methylene blue

Primary stain used for acid fast staining

Carbol fushin

Hemolysis type of bacterial culture that exihibit Greenish or brownish discoloration

Alpha hemolyis

Hemolysis type of bacterial culture that exihibit Clear zone around the colony

Beta hemolysis

Hemolysis type of bacterial culture that exihibit No hemolysis

Gamma hemolyis

Medium used best for turbidity test

Nutrient Broth (NB)

Medium used as a base medium for Blood Agar

Tryptic soy Agar (TSA)

Medium normally used for blood culture bottles

Brain heart infusion Agar (BHI agar)

Medium best used for differentiation of hemolytic pattern

Blood Agar (BA/BAP)

Medium that produce Factor X and Factor V

Chocolate Agar (CA/CAP)

Medium that favor the growth of Vibrio

Alkaline peptone water (APW)

Medium That favor the growth of salmonella

Selenite F broth

Medium that favor the growth of Salmonella and shigella

Gram negative Broth (GN)

Medium that promotes the growth of Gram negative enteric bacilli

Eosin-methelyne blue (EMB) agar

Fermenter or non Fermenter : EMB with dark violet colonies

Fermenter

Agar that favors the growth of gram negative Enteric bacilli, differentiate Hydrogen sulfide producers

Hektoen enteric agar

Agar that favors the growth of gram negative Enteric bacilli, differentiate the lactose and non lactose fermenter produce dark violet colonies to fermenters

Eosin methylene blue Agar (EMB)

Agar that favors the growth of gram negative Enteric bacilli, and produces pink colonies to lactose fermenters

Mc Conckey agar ( MAC )

Agar that differentiate Shigella and Salmonella

Salmonella-Shigella Agard (SSA)

Agar that highly selective for Salmonella Typhi

Bismuth sulfite Agar (BSA)

Agar that favor the growth of salmonella spp. differentiate sucrose fermenter and h2s producers

Brilliant green agar (BGA)

Agar that highly selective for Vibrio spp. differentiate Sucrose fermenter and hydrogen sulfide producers

Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)

Agar that selective for Staphylococcus spp Differentiate manitol fermenters

Mannitol Salt Agar (MSA)

EMB (-) Lactose fermenter

Colorless

MAC (-) for lactose fermenter

Colorless

Mac (+) for lactose fermenter

Pink

Hea (+) For lactose fermenter (-) for H2s

Orange without blackening

Hea (+) For lactose fermenter (+) for h2s

Orange with blackening

Hea (-) lactose fermenter (-) for H2s

Blue-green without blackening

BGA (+) for lactose-sucrose fermenter

yellow green

BGA (-) for lactose-sucrose fermenter

Red - Pinkish white

Tcbs result : Blue green without blackening

Negative sucrose fermenter , negative h2s production

Tcbs result : yellow without blackening

Postivie sucrose fermenter , negative h2s production

MSA (+) Manitol fermenter

Yellow

MSA (-) Manitol fermenter

Dark pink

Positive result for Indole in SIM

Pink ring

Sulfide - indole motility medium :

Medium produced a Pink ring without blackening and Growth are within the stabline

Indole (+)
h2s (-)
Motility (-)

Sulfide - indole motility medium :

Medium produced a Blackening in the medium Growth are within the Stabline

Indole (-)
h2s (+)
Motility (-)

Sulfide - indole motility medium :

Medium produced a Pink ring with blackening and growth spreading from the stabline

Indole (+)
h2s (+)
Motility (+)

MR test postive result

Red

Vp test Positive result

Red

Vp test negative result

No color change

Mr test negative result

yellow

SCA test (+)

Blue

SCA test (-)

Green

Which organism fit this description
Indole +
MR +
VP -
SCA -

E. coli

Which organism fit this description
Indole -
MR +
VP -
SCA -

Shigella

Which organism fit this description
Indole -
MR +
VP -
SCA +

Salmonella

Which organism fit this description
Indole +
MR +
VP -
SCA +

Proteus vulgaris

Which organism fit this description
Indole -
MR -
VP +
SCA +

Klebsiella pneumoniae

Results on LIA for Deaminase + and Decarboxylase -

R/A

Results on LIA for Deaminase - and Decarboxylase -

K/A

Results on LIA for Deaminase - and Decarboxylase +

K/K

Result on TSI for Fermentation of Glucose only

K/A

Result on TSI for No fermentation

K/K

Result on TSI for Fermentation of Glucose and Lactose/Sucrose

A/A

Stuarts urea broth : detects urease production what is the color of the result

Red

Negative Urease production on Stuarts urea broth

Yellow-orange / no color change

Postive result for Moellers broth

Purple

Negative Result for Moellers broth

Yellow