The Microscope

What are the three main things microscopes use to get a clear image of a specimen?

Magnification, resolution, and contrast.

What does it mean if a microscope is parfocal?

It remains in focus when switching between objective lenses.

How do you calculate the total magnification of a specimen?

Multiply the objective lens magnification by the ocular lens magnification.

Why does a microscopic image appear inverted?

Because light passes through two lens systems and is refracted, causing the rays to converge into an enlarged, inverted image.

What is resolution in microscopy?

The ability of a lens to separate or distinguish between small objects that are close together.

What is the resolving power of a light microscope using an oil immersion lens?

0.2 μm (micrometers).

What does Refractive Index (RI) measure?

The deflection of a light ray from a straight path as it travels from one medium (like air) to another (like glass).