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which agonist stimulates intestinal smooth muscle to contract
acetylcholine
bind reversibly with muscarinic acetylcholine receptors
ec50
the concentration of agonist that produces 50% of the maximum possible response
IC50
the concentration of inhibitor/antagonist needed to inhibit reaction by 50%
why is co2 and oxygen bubbled through solution
to maintain a constant pH
supply cells with oxygen
ensure rapid mixing of the organ bath