Practical Info

Enumerate only

peptone water

* Composition
* Sterilization
* Uses

* 1% peptone + .5% NaCl
* autoclave
* base for
* Sugar media
* indole production test
* Nutrient Broth

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Nutrient broth

* Type of media
* Method of sterilization
* Composition
* Ph
* uses

* Simple
* Autoclave
* Peptone Water + Meat extract
* 7.4
* INCREASE number of microorganism

\
Nutrient slope

* Type of media
* Uses
* Method of sterilization
* Composition

* simple
* Preservation of microorganisms for long time
* Autoclave
* Nutrient Broth + agar agar

Nutrient Agar

* Type of media
* Uses
* Method of sterilization
* Composition
* Alternative media

* Simple
* \

1. Cultivation of Staphylococci to show Endo pigment

\---- ---------------------------------------

Aureus →orange

Albicans → White

Citrus → yellow

\--------------------------


2. Cultivation of Pseudomonas which gives Exopigement

\----------------------------------
3. proteus --→ wavy colonies

\-----------------------------------
4. Anthracoid -→ Medusa head appearance

* Autoclave
* Nutrient Broth + agar agar
* Milk Media

blood agar

* type of media
* media Uses
* Method of sterilization
* Composition

* Enriched
* Cultivation of

Staph.aureus → beta /complete

Pneumococcal → ALPHA

streptococcus Fecalis -→ Jama

\----------------------------------

* Nutrient agar →Autoclave → blood already sterile
* Blood + nutrient agar

chocolate agar

* type of media
* Uses
* Method of sterilization
* Composition

* enriched
* Cultivation of Pneumococci
* Nutrient agar → autoclave , blood is sterile
* Blood + Nutrient Agar

Milk Agar

* type of media
* Uses
* Method of sterilization
* Composition

* Enriched
* Cultivation of Staph.spp
* Nutrient agar → Autoclave , milk pasteurization
* Milk + Nutrient Agar

lofflers serum

* type of media
* media Uses
* Method of sterilization
* Composition:
* Alternative media

* Enriched
* Cultivation of Corynobacteria Diphteria
* Inspissation
* 3 part of sterile human serum + 1part of nutrient broth
* Blood Tellurite

dorset egg medium

* type of media
* Uses
* Method of sterilization
* Composition
* Alternative media

* Enriched
* Cultivation of TB (mycobacterium tuberculosis)
* Inspissation
* 3 Parts of egg + 1 part of Distilled water
* Lowenstein Jensen Media

Lowenstein jensen

* type of media
* Uses
* Method of sterilization
* Composition
* Alternative media

* Selective Media
* Cultivation of TB
* Inspissation
* Egg+ Mineral salts + Malachite Green (selective substance)
* Dorset Egg

thiosulphate citrate bile slat sucrose (TCBS)

* type of media
* Uses
* Method of sterilization
* Composition
* selective-indicator substances

* Selective
* Cultivation of Cholera
* Tyndlization
* Thiosulphate + Citrate +Bile salts + Sucrase +Thymol Blue + Bromothymol Blue
* Bile salt ( selective media) -Thymol blue+bromothymol blue (indicator substance)

Selective & indicative media

TCBS

MacConkey agar

* type of media
* Uses
* Method of sterilization
* Composition
* Alternative media

* Differential
* Cultivation of Salmonella, E.coli

\--------------------------------
* SAlmonella → Pale Yellow
* E.coli → Pink

\--------------------------------
* Tyndlization
* Piptone Water + Agar + Bile Salt + Lactose + Neutral red
* Selective substance: bile salt Indicator: ( neutral red)
* Triple Sugar Iron (Salmonella), EMB(E.coli)

triple sugar iron

* type of media
* Uses
* Method of sterilization
* Composition:
* indicator substance

* Differential
* Cultivation of
* Proteus → Dark red
* Pseudomonas → Bright red
* E.coli → Brown
* Salmonella
* Tyndlization
* glucose+ lactose+ sucrose +ferrous sulphate+ beef and yeast extract+ agar+ phenol red
* phenol red

bismuth sulphite agar

* type of media
* Uses:
* Method of sterilization :
* Composition:
* Selective substance and indicator

* Differential
* Cultivation of Salmonella
* Autoclave
* Bismuth Sulphite + Agar + Brilliant Green
* Brilliant green

eosin methylene blue (EMB)

* type of media
* Uses
* Method of sterilization
* Composition
* Alternative Media

* Differential
* Cultivation of Klebsiella, E.coli
* Tyndlization
* Eosin + Methylene + Lactose
* MacConkey

Mannitol salt agar

* type of media
* Uses
* Method of sterilization
* composition
* Indicator: , Selective substance :
* Alternitve media r

* Differential
* Cultivation of Staph Aures
* Tyndlization
* Mannitol + 7.5% Nacl +Phenol Red + Agar
* Indicator → Phenol Red SS→ NaCl
* Blood Agar

simmon citrate medium

* type of media:
* Uses
* Method of sterilization
* Composition
* Alternative media

* Differential
* Cultivation of

\------------------------

E.coli → citrate -ve

Klebsilla, citrobactor → citrate +ve

\--------------------------
* Tyndlization
* Citrate + Sugar + Bromothymol Blue (Indicator)
* MacConkey

deep agar

* type of media
* Uses
* Method of sterilization :
* Composition:

* Simple
* Cultivation of Anaerobic Cultivation
* Autocalve
* Nutrient Broth + agar agar

thioglycolate media (fluid media)

* type of media:
* Uses:
* Method of sterilization
* Reducing substance

* Simple Media
* Cultivation of Anerobic Microorganism
* Autoclave
* Na Thioglycolate

\

Robertson cooked meat

* Type of media :
* Uses:
* Method of sterilization
* Reducing substance

* Simple Media
* Cultivation of anaerobic organism
* Autoclave
* Haemitin + Glutathione

\: Sabouraud’s dextrose agar

* type of media:
* composition
* sterilisation:
* use:
* selective substance

* Selective
* Peptone + agar + glucose + Gentamycin + Chloramphenicol+ Cycloheximide
* Tyndlization
* Candida Cultivation
* Gentamycin + Chloramphenicol

Blood Tellurite Medium

* type
* Composition
* Sterlization
* uses

* Slecetive
* Blood agar + ,03% potassium tellurite (SS)
* Autoclave + already strile
* Diphetria
* ^^^enrished selective differential media “““

Sugar media

* type
* Composition
* uses
* Sterlization

* Enrichment
* Peptone water + test sugar + 1% andrades indicator (Colorless in alkaline, red in acidic)
* identification of bacteria
* Tyndillization

Types of Media

* simple
* enriched
* selective
* Differential
* Enrichment
* Sugar media

Simple media

* Fluid

- Peptone water

-Nutrient Broth

* Solid → nutrient agar

Enriched media

* blood agar
* Chocolate agar
* milk
* loffler
* dorset

Differential media

* MacConkey
* TSI
* EMB
* Bismuth Sulphate
* Mannitol agar
* simmon Citrate
* Christensen urea

Christensen urea

* type
* Composition
* Sterlization
* uses

* differential
* Urea agar + phenol agar
* autocalve - filteration
* proteus