Enumerate only
peptone water
Composition
Sterilization
Uses
1% peptone + .5% NaCl
autoclave
base for
Sugar media
indole production test
Nutrient Broth
Nutrient broth
Type of media
Method of sterilization
Composition
Ph
uses
Simple
Autoclave
Peptone Water + Meat extract
7.4
INCREASE number of microorganism
Nutrient slope
Type of media
Uses
Method of sterilization
Composition
simple
Preservation of microorganisms for long time
Autoclave
Nutrient Broth + agar agar
Nutrient Agar
Type of media
Uses
Method of sterilization
Composition
Alternative media
Simple
Cultivation of Staphylococci to show Endo pigment
---- ---------------------------------------
Aureus →orange
Albicans → White
Citrus → yellow
--------------------------
Cultivation of Pseudomonas which gives Exopigement
----------------------------------
proteus --→ wavy colonies
-----------------------------------
Anthracoid -→ Medusa head appearance
Autoclave
Nutrient Broth + agar agar
Milk Media
blood agar
type of media
media Uses
Method of sterilization
Composition
Enriched
Cultivation of
Staph.aureus → beta /complete
Pneumococcal → ALPHA
streptococcus Fecalis -→ Jama
----------------------------------
Nutrient agar →Autoclave → blood already sterile
Blood + nutrient agar
chocolate agar
type of media
Uses
Method of sterilization
Composition
enriched
Cultivation of Pneumococci
Nutrient agar → autoclave , blood is sterile
Blood + Nutrient Agar
Milk Agar
type of media
Uses
Method of sterilization
Composition
Enriched
Cultivation of Staph.spp
Nutrient agar → Autoclave , milk pasteurization
Milk + Nutrient Agar
lofflers serum
type of media
media Uses
Method of sterilization
Composition:
Alternative media
Enriched
Cultivation of Corynobacteria Diphteria
Inspissation
3 part of sterile human serum + 1part of nutrient broth
Blood Tellurite
dorset egg medium
type of media
Uses
Method of sterilization
Composition
Alternative media
Enriched
Cultivation of TB (mycobacterium tuberculosis)
Inspissation
3 Parts of egg + 1 part of Distilled water
Lowenstein Jensen Media
Lowenstein jensen
type of media
Uses
Method of sterilization
Composition
Alternative media
Selective Media
Cultivation of TB
Inspissation
Egg+ Mineral salts + Malachite Green (selective substance)
Dorset Egg
thiosulphate citrate bile slat sucrose (TCBS)
type of media
Uses
Method of sterilization
Composition
selective-indicator substances
Selective
Cultivation of Cholera
Tyndlization
Thiosulphate + Citrate +Bile salts + Sucrase +Thymol Blue + Bromothymol Blue
Bile salt ( selective media) -Thymol blue+bromothymol blue (indicator substance)
Selective & indicative media
TCBS
MacConkey agar
type of media
Uses
Method of sterilization
Composition
Alternative media
Differential
Cultivation of Salmonella, E.coli
--------------------------------
SAlmonella → Pale Yellow
E.coli → Pink
--------------------------------
Tyndlization
Piptone Water + Agar + Bile Salt + Lactose + Neutral red
Selective substance: bile salt Indicator: ( neutral red)
Triple Sugar Iron (Salmonella), EMB(E.coli)
triple sugar iron
type of media
Uses
Method of sterilization
Composition:
indicator substance
Differential
Cultivation of
Proteus → Dark red
Pseudomonas → Bright red
E.coli → Brown
Salmonella
Tyndlization
glucose+ lactose+ sucrose +ferrous sulphate+ beef and yeast extract+ agar+ phenol red
phenol red
bismuth sulphite agar
type of media
Uses:
Method of sterilization :
Composition:
Selective substance and indicator
Differential
Cultivation of Salmonella
Autoclave
Bismuth Sulphite + Agar + Brilliant Green
Brilliant green
eosin methylene blue (EMB)
type of media
Uses
Method of sterilization
Composition
Alternative Media
Differential
Cultivation of Klebsiella, E.coli
Tyndlization
Eosin + Methylene + Lactose
MacConkey
Mannitol salt agar
type of media
Uses
Method of sterilization
composition
Indicator: , Selective substance :
Alternitve media r
Differential
Cultivation of Staph Aures
Tyndlization
Mannitol + 7.5% Nacl +Phenol Red + Agar
Indicator → Phenol Red SS→ NaCl
Blood Agar
simmon citrate medium
type of media:
Uses
Method of sterilization
Composition
Alternative media
Differential
Cultivation of
------------------------
E.coli → citrate -ve
Klebsilla, citrobactor → citrate +ve
--------------------------
Tyndlization
Citrate + Sugar + Bromothymol Blue (Indicator)
MacConkey
deep agar
type of media
Uses
Method of sterilization :
Composition:
Simple
Cultivation of Anaerobic Cultivation
Autocalve
Nutrient Broth + agar agar
thioglycolate media (fluid media)
type of media:
Uses:
Method of sterilization
Reducing substance
Simple Media
Cultivation of Anerobic Microorganism
Autoclave
Na Thioglycolate
Robertson cooked meat
Type of media :
Uses:
Method of sterilization
Reducing substance
Simple Media
Cultivation of anaerobic organism
Autoclave
Haemitin + Glutathione
: Sabouraud’s dextrose agar
type of media:
composition
sterilisation:
use:
selective substance
Selective
Peptone + agar + glucose + Gentamycin + Chloramphenicol+ Cycloheximide
Tyndlization
Candida Cultivation
Gentamycin + Chloramphenicol
Blood Tellurite Medium
type
Composition
Sterlization
uses
Slecetive
Blood agar + ,03% potassium tellurite (SS)
Autoclave + already strile
Diphetria
^^^enrished selective differential media “““
Sugar media
type
Composition
uses
Sterlization
Enrichment
Peptone water + test sugar + 1% andrades indicator (Colorless in alkaline, red in acidic)
identification of bacteria
Tyndillization
Types of Media
simple
enriched
selective
Differential
Enrichment
Sugar media
Simple media
Fluid
- Peptone water
-Nutrient Broth
Solid → nutrient agar
Enriched media
blood agar
Chocolate agar
milk
loffler
dorset
Differential media
MacConkey
TSI
EMB
Bismuth Sulphate
Mannitol agar
simmon Citrate
Christensen urea
Christensen urea
type
Composition
Sterlization
uses
differential
Urea agar + phenol agar
autocalve - filteration
proteus