Practical Info

Enumerate only

peptone water

• Composition

• Sterilization

• Uses

peptone water

• Composition

• Sterilization

• Uses

• 1% peptone + .5% NaCl

• autoclave

• base for

  • Sugar media

  • indole production test

  • Nutrient Broth

Nutrient broth

• Type of media

• Method of sterilization

• Composition

• Ph

• uses

• Simple

• Autoclave

• Peptone Water + Meat extract

• 7.4

• INCREASE number of microorganism

Nutrient slope

• Type of media

• Uses

• Method of sterilization

• Composition

• simple

• Preservation of microorganisms for long time

• Autoclave

• Nutrient Broth + agar agar

Nutrient Agar

• Type of media

• Uses

• Method of sterilization

• Composition

• Alternative media

• Simple

• 1. Cultivation of Staphylococci to show Endo pigment

---- ---------------------------------------

Aureus →orange

Albicans → White

Citrus → yellow

--------------------------

2. Cultivation of Pseudomonas which gives Exopigement

   ----------------------------------

3. proteus --→ wavy colonies

   -----------------------------------

4. Anthracoid -→ Medusa head appearance

• Autoclave

• Nutrient Broth + agar agar

• Milk Media

blood agar

• type of media

• media Uses

• Method of sterilization

• Composition

• Enriched

• Cultivation of

  Staph.aureus → beta /complete

  Pneumococcal → ALPHA

  streptococcus Fecalis -→ Jama

----------------------------------

• Nutrient agar →Autoclave → blood already sterile

• Blood + nutrient agar

chocolate agar

• type of media

• Uses

• Method of sterilization

• Composition

• enriched

• Cultivation of Pneumococci

• Nutrient agar → autoclave , blood is sterile

• Blood + Nutrient Agar

Milk Agar

• type of media

• Uses

• Method of sterilization

• Composition

• Enriched

• Cultivation of Staph.spp

• Nutrient agar → Autoclave , milk pasteurization

• Milk + Nutrient Agar

lofflers serum

• type of media

• media Uses

• Method of sterilization

• Composition:

• Alternative media

• Enriched

• Cultivation of Corynobacteria Diphteria

• Inspissation

• 3 part of sterile human serum + 1part of nutrient broth

• Blood Tellurite

dorset egg medium

• type of media

• Uses

• Method of sterilization

• Composition

• Alternative media

• Enriched

• Cultivation of TB (mycobacterium tuberculosis)

• Inspissation

• 3 Parts of egg + 1 part of Distilled water

• Lowenstein Jensen Media

Lowenstein jensen

• type of media

• Uses

• Method of sterilization

• Composition

• Alternative media

• Selective Media

• Cultivation of TB

• Inspissation

• Egg+ Mineral salts + Malachite Green (selective substance)

• Dorset Egg

thiosulphate citrate bile slat sucrose (TCBS)

• type of media

• Uses

• Method of sterilization

• Composition

• selective-indicator substances

• Selective

• Cultivation of Cholera

• Tyndlization

• Thiosulphate + Citrate +Bile salts + Sucrase +Thymol Blue + Bromothymol Blue

• Bile salt ( selective media) -Thymol blue+bromothymol blue (indicator substance)

Selective & indicative media

TCBS

MacConkey agar

• type of media

• Uses

• Method of sterilization

• Composition

• Alternative media

• Differential

• Cultivation of Salmonella, E.coli

  --------------------------------

• SAlmonella → Pale Yellow

• E.coli → Pink

  --------------------------------

• Tyndlization

• Piptone Water + Agar + Bile Salt + Lactose + Neutral red

• Selective substance: bile salt Indicator: ( neutral red)

• Triple Sugar Iron (Salmonella), EMB(E.coli)

triple sugar iron

• type of media

• Uses

• Method of sterilization

• Composition:

• indicator substance

• Differential

• Cultivation of

  • Proteus → Dark red

  • Pseudomonas → Bright red

  • E.coli → Brown

  • Salmonella

• Tyndlization

• glucose+ lactose+ sucrose +ferrous sulphate+ beef and yeast extract+ agar+ phenol red

• phenol red

bismuth sulphite agar

• type of media

• Uses:

• Method of sterilization :

• Composition:

• Selective substance and indicator

• Differential

• Cultivation of Salmonella

• Autoclave

• Bismuth Sulphite + Agar + Brilliant Green

• Brilliant green

eosin methylene blue (EMB)

• type of media

• Uses

• Method of sterilization

• Composition

• Alternative Media

• Differential

• Cultivation of Klebsiella, E.coli

• Tyndlization

• Eosin + Methylene + Lactose

• MacConkey

Mannitol salt agar

• type of media

• Uses

• Method of sterilization

• composition

• Indicator: , Selective substance :

• Alternitve media r

• Differential

• Cultivation of Staph Aures

• Tyndlization

• Mannitol + 7.5% Nacl +Phenol Red + Agar

• Indicator → Phenol Red SS→ NaCl

• Blood Agar

simmon citrate medium

• type of media:

• Uses

• Method of sterilization

• Composition

• Alternative media

• Differential

• Cultivation of

  ------------------------

  E.coli → citrate -ve

  Klebsilla, citrobactor → citrate +ve

  --------------------------

• Tyndlization

• Citrate + Sugar + Bromothymol Blue (Indicator)

• MacConkey

deep agar

• type of media

• Uses

• Method of sterilization :

• Composition:

• Simple

• Cultivation of Anaerobic Cultivation

• Autocalve

• Nutrient Broth + agar agar

thioglycolate media (fluid media)

• type of media:

• Uses:

• Method of sterilization

• Reducing substance

• Simple Media

• Cultivation of Anerobic Microorganism

• Autoclave

• Na Thioglycolate

Robertson cooked meat

• Type of media :

• Uses:

• Method of sterilization

• Reducing substance

• Simple Media

• Cultivation of anaerobic organism

• Autoclave

• Haemitin + Glutathione

: Sabouraud’s dextrose agar

• type of media:

• composition

• sterilisation:

• use:

• selective substance

• Selective

• Peptone + agar + glucose + Gentamycin + Chloramphenicol+ Cycloheximide

• Tyndlization

• Candida Cultivation

• Gentamycin + Chloramphenicol

Blood Tellurite Medium

• type

• Composition

• Sterlization

• uses

• Slecetive

• Blood agar + ,03% potassium tellurite (SS)

• Autoclave + already strile

• Diphetria

• ^^^enrished selective differential media “““

Sugar media

• type

• Composition

• uses

• Sterlization

• Enrichment

• Peptone water + test sugar + 1% andrades indicator (Colorless in alkaline, red in acidic)

• identification of bacteria

• Tyndillization

Types of Media

• simple

• enriched

• selective

• Differential

• Enrichment

• Sugar media

Simple media

• Fluid

  - Peptone water

  -Nutrient Broth

• Solid → nutrient agar

Enriched media

• blood agar

• Chocolate agar

• milk

• loffler

• dorset

Differential media

• MacConkey

• TSI

• EMB

• Bismuth Sulphate

• Mannitol agar

• simmon Citrate

• Christensen urea

Christensen urea

• type

• Composition

• Sterlization

• uses

• differential

• Urea agar + phenol agar

• autocalve - filteration

• proteus