Chapter 13 & 14 DNA Fingerprinting

Gel Electrophoresis

The technique by which DNA is separated based on length using electricity

Do shorter or longer DNA fragments travel further in the gel?

Shorter DNA fragments

What is the preferred medium used in DNA fingerprinting and Gel Electrophoresis

Agarose Gel

DNA fingerprinting is used to the the DNA ________/DNA profile of someone

fingerprint

What technique is used to find the identity of someone using DNA samples?

DNA Fingerprinting

Proteins that cut DNA into small fragments. Examples include EcoRI and PstI

Restriction Enzymes

Device used to deliver very small amounts of liquids and is calibrated in μl

Micropipette

Label all the parts of a micropipette

What colored tip is used by the 10 μl Micropipettes

Clear

What colored tip is used by the 20 μl Micropipettes

Yellow

What charge is DNA

Negative

Steps of DNA Fingerprinting and Gel Electrophoresis

1. Add Restriction Enzyme to each DNA sample

2. Place DNA samples into the Centrifuge

3. Place the samples in a Water bath

4. Pour and set up the Gel Tray

5. Load and Run Gel through the a current using a PowerPac

6. Stain the Gel

Do you change pipette tips after every use? (T/F)

True

How does Gel Electrophoresis organize DNA according to size?

Through electricity

What is a real world application for gel electrophoresis?

DNA Fingerprinting

to find identity of people for crimes or to identify a body

What is this

Micropipette tip

What is this

Powerpac

What is this

Electrophoresis Gel Chamber

What is this

Centrifuge

What is this and its parts

Gel Tray