unusual gram neg rods- ella sisters

pasteurella maltocida normal flora

wild and domestic animals

where is pasteurella maltocida commonly isolated from in humans

dog and cat bites or scratches

pasteurella maltocida is indole, catalase, and oxidase

positive

pasteurella maltocida gram stain appearance

pleomorphic, bipolar safety pin, can be coccobacillary

franciscella tularensis transmission

• rabbits and other rodents

• cats if they eat wild rodents

• insect and tick bites

does franciscella tularensis spread from person to person

NO only from animal to person

zoonoses of the unusual GNR

all but bordetella

agents of bioterrorism

• franciscella tularensis

• brucella

franciscella tularensis tularemia forms

• skin lesions- nodules, from skinning rabbits

• ocular

• pulmonary

franciscella tularensis treatment

bactericidal drugs

franciscella tularensis specimens of choice

• ulcer fluid

• blood

franciscella tularensis media

• blood with cystine

• BCYEA

• chocolate with extra isovitalex

• CHAB

CHAB agar is

selective for franciscella tularensis

franciscella tularensis growth rate

10-14 days

franciscella tularensis is catalse and beta lactamase

pos

franciscella tularensis is urease and oxidase

neg

franciscella tularensis gram stain

red, round, and random

franciscella tularensis serological diagnosis

used for conformation

franciscella tularensis titer

• 4 fold increase

• single titer of 1:160 or more

brucella disease

brucellosis (undulant fever)

brucella abortus

cows

brucella mlitensis

goats

brucella canis

dogs

brucella suis

pigs

brucella animal disease

spontaneous abortion

brucella transmission to humans

unpasteurized dairy products

brucella specimen of choice

blood

how long do you keep brucella blood clutures for?

30 days

selective media for brucella

BCYEA

brucella serology conformation

• 4 fold rise

• >100 IU single titer

bordetella pertussis

whooping cough

bordetella pertussis transport media

regan-lowe with charcoal, horse blood, and antibiotics

bordetella pertussis specimen of choice

NP swab NOT throat

bordetella pertussis traditional media

bordet-gengou

bordetella pertussis modern media

regan-lowe

bordetella pertussis colony morphology

resemble droplets of mercury

bordetella pertussis gram stain

small uniform GNR, slow to counterstain

bordetella parapertussis

• milder than pertussis

• URT, cough

bordetella bronchiseptica diseases

• kennel cough in animals

• human infections- dog bite wounds, bronchitis, pneumonia

bordetella avium diseases

• not pathogenic to humans

• URT infections in turkeys

eikenella corrocdens normal flora

human mucous membranes especially the mouth

eikenella corrodens lab characteristics

• bleach smell

• pits agar